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Literature DB >> 2478533

Probing the structure of the mitochondrial channel, VDAC, by site-directed mutagenesis: a progress report.

E Blachly-Dyson¹, S Z Peng, M Colombini, M Forte.

Abstract

The voltage-dependent anion-selective channel (VDAC) of the mitochondrial outer membrane is formed by a small (approximately 30 kDa) polypeptide, but shares with more complex channels the properties of voltage-dependent gating and ion selectivity. Thus, it is a useful model for studying these properties. The molecular biology techniques available in yeast allow us to construct mutant versions of the cloned yeast VDAC gene in vitro, using oligonucleotide-directed mutagenesis, and to express the mutant genes in yeast cells in the absence of wild-type VDAC. We find that one substitution mutation (lys 61 to glu) alters the selectivity of VDAC.

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Year: 1989 PMID： 2478533 DOI： 10.1007/bf00762519

Source DB: PubMed Journal: J Bioenerg Biomembr ISSN： 0145-479X Impact factor: 2.945

25 in total

Review 1. Molecular genetics of the VDAC ion channel: structural model and sequence analysis.

Authors: M Forte; H R Guy; C A Mannella
Journal: J Bioenerg Biomembr Date: 1987-08 Impact factor: 2.945

2. Voltage-dependent channels found in the membrane fraction of corn mitochondria.

Authors: D P Smack; M Colombini
Journal: Plant Physiol Date: 1985-12 Impact factor: 8.340

3. Import of proteins into mitochondria. In vitro studies on the biogenesis of the outer membrane.

Authors: S M Gasser; G Schatz
Journal: J Biol Chem Date: 1983-03-25 Impact factor: 5.157

4. Isolation and properties of the porin of the outer mitochondrial membrane from Neurospora crassa.

Authors: H Freitag; R Benz; W Neupert
Journal: Methods Enzymol Date: 1983 Impact factor: 1.600

5. A simple method for displaying the hydropathic character of a protein.

Authors: J Kyte; R F Doolittle
Journal: J Mol Biol Date: 1982-05-05 Impact factor: 5.469

6. Reconstitution in planar lipid bilayers of a voltage-dependent anion-selective channel obtained from paramecium mitochondria.

Authors: S J Schein; M Colombini; A Finkelstein
Journal: J Membr Biol Date: 1976-12-28 Impact factor: 1.843

7. Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.

Authors: D Shortle; P Grisafi; S J Benkovic; D Botstein
Journal: Proc Natl Acad Sci U S A Date: 1982-03 Impact factor: 11.205

8. Purification and characterisation of a pore protein of the outer mitochondrial membrane from Neurospora crassa.

Authors: H Freitag; W Neupert; R Benz
Journal: Eur J Biochem Date: 1982-04

9. A yeast mutant lacking mitochondrial porin is respiratory-deficient, but can recover respiration with simultaneous accumulation of an 86-kd extramitochondrial protein.

Authors: M Dihanich; K Suda; G Schatz
Journal: EMBO J Date: 1987-03 Impact factor: 11.598

10. Molecular cloning and sequencing of cDNA for yeast porin, an outer mitochondrial membrane protein: a search for targeting signal in the primary structure.

Authors: K Mihara; R Sato
Journal: EMBO J Date: 1985-03 Impact factor: 11.598

16 in total

Probing the structure of the mitochondrial channel, VDAC, by site-directed mutagenesis: a progress report.

Review 1. Molecular genetics of the VDAC ion channel: structural model and sequence analysis.

2. Voltage-dependent channels found in the membrane fraction of corn mitochondria.

3. Import of proteins into mitochondria. In vitro studies on the biogenesis of the outer membrane.

4. Isolation and properties of the porin of the outer mitochondrial membrane from Neurospora crassa.

5. A simple method for displaying the hydropathic character of a protein.

6. Reconstitution in planar lipid bilayers of a voltage-dependent anion-selective channel obtained from paramecium mitochondria.

7. Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.

8. Purification and characterisation of a pore protein of the outer mitochondrial membrane from Neurospora crassa.

9. A yeast mutant lacking mitochondrial porin is respiratory-deficient, but can recover respiration with simultaneous accumulation of an 86-kd extramitochondrial protein.

10. Molecular cloning and sequencing of cDNA for yeast porin, an outer mitochondrial membrane protein: a search for targeting signal in the primary structure.

1. Indications of a common folding pattern for VDAC channels from all sources.

Review 2. Toward the molecular structure of the mitochondrial channel, VDAC.

3. VDAC: the channel at the interface between mitochondria and the cytosol.

4. Protein engineering and site-directed mutagenesis. Patents and literature.

5. Modulation of plant mitochondrial VDAC by phytosterols.

Review 6. Structural analysis of mitochondrial pores.

7. Mapping of residues forming the voltage sensor of the voltage-dependent anion-selective channel.

8. Oriented channel insertion reveals the motion of a transmembrane beta strand during voltage gating of VDAC.

9. Zero-current potentials in a large membrane channel: a simple theory accounts for complex behavior.

Review 10. Structure of the mitochondrial outer membrane channel derived from electron microscopy of 2D crystals.