| Literature DB >> 24780640 |
N S Singh1, K-L Habicht2, K S S Dossou1, R Shimmo2, I W Wainer1, R Moaddel3.
Abstract
Cellular membrane affinity chromatography stationary phases have been extensively used to characterize immobilized proteins and provide a direct measurement of multiple binding sites, including orthosteric and allosteric sites. This review will address the utilization of immobilized cellular and tissue fragments to characterize multiple transmembrane proteins co-immobilized onto a stationary phase. This approach will be illustrated by demonstrating that multiple transmembrane proteins were immobilized from cell lines and tissue fragments. In addition, the immobilization of individual compartments/organelles within a cell will be discussed and the changes in the proteins binding/kinetics based on their location. Published by Elsevier B.V.Entities:
Keywords: ATP-binding cassette transporters; Bioaffinity chromatography; G-protein coupled receptors; Ligand-gated ion channels
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Year: 2014 PMID： 24780640 PMCID： PMC4127356 DOI： 10.1016/j.jchromb.2014.04.005
Source DB: PubMed Journal: J Chromatogr B Analyt Technol Biomed Life Sci ISSN： 1570-0232 Impact factor: 3.205