Literature DB >> 2477175

Total protein determined in human breast milk by use of coomassie brilliant blue and centrifugal analysis.

Y Bergqvist1, L Karlsson, L Fohlin.   

Abstract

This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.

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Year:  1989        PMID： 2477175

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  3 in total

1.  Establishment of micromethods for macronutrient contents analysis in breast milk.

Authors:  Arum Choi; Gerhard Fusch; Niels Rochow; Natasha Sheikh; Christoph Fusch
Journal:  Matern Child Nutr       Date:  2013-06-18       Impact factor: 3.092

2.  Comparison of macronutrient content in human milk measured by mid-infrared human milk analyzer and reference methods.

Authors:  Francesca Giuffrida; Sean Austin; Denis Cuany; Belén Sanchez-Bridge; Karin Longet; Emmanuelle Bertschy; Julien Sauser; Sagar K Thakkar; Le Ye Lee; Michael Affolter
Journal:  J Perinatol       Date:  2018-12-14       Impact factor: 2.521

3.  Comparative study of the most commonly used methods for total protein determination in milk of different species and their ultrafiltration products.

Authors:  Diego Hueso; Javier Fontecha; Pilar Gómez-Cortés
Journal:  Front Nutr       Date:  2022-09-13
  3 in total

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