| Literature DB >> 2476805 |
C Dingwall1, I Ernberg, M J Gait, S M Green, S Heaphy, J Karn, A D Lowe, M Singh, M A Skinner, R Valerio.
Abstract
tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes. Purified tat binds specifically to HIV-1 trans-activation-responsive region (TAR) RNA in gel-retardation, filter-binding, and immunoprecipitation assays. tat does not bind detectably to antisense TAR RNA sequences, cellular mRNA sequences, variant TAR RNA sequences with altered stem-loop structures, or TAR DNA.Entities:
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Year: 1989 PMID: 2476805 PMCID: PMC297963 DOI: 10.1073/pnas.86.18.6925
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN: 0027-8424 Impact factor: 11.205