Literature DB >> 24767682

Evaluation of the RIDAGENE real-time PCR assay for the detection of GI and GII norovirus.

N L Dunbar1, L D Bruggink1, J A Marshall2.   

Abstract

The current study examined the efficacy of the RIDAGENE norovirus (NoV) real-time polymerase chain reaction assay (R-Biopharm, Darmstadt, Germany) for use in a routine diagnostic laboratory. The RIDAGENE assay had an overall sensitivity of 98% but was more sensitive for GII than GI NoV. The assay had a specificity of 98%. The RIDAGENE assay could detect a variety of GI and GII open reading frame 2 genotypes including GI.1, GI.3, GI.8, GI.13, GII.2, GII.3, GII.4 (including the following variants: 2006b, 2009, 2012, and 3 others that have not been assigned), GII.6, GII.12, and GII.13. The assay did not cross react with a number of gastroenteritis viruses including adenovirus, astrovirus, rotavirus, and sapovirus. The assay was straightforward to perform, and for a run of 50 specimens, a result was obtainable in roughly 4 hours. The RIDAGENE assay can be recommended as a valuable detection method for NoV.
Copyright © 2014 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Norovirus; Real-time PCR

Mesh：

Year:  2014        PMID： 24767682     DOI： 10.1016/j.diagmicrobio.2014.03.017

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  8 in total

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Authors:  E Aho-Laukkanen; J J Hirvonen; K Saha
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2017-01-06       Impact factor: 3.267

Review 2.  Norovirus.

Authors:  Elizabeth Robilotti; Stan Deresinski; Benjamin A Pinsky
Journal:  Clin Microbiol Rev       Date:  2015-01       Impact factor: 26.132

3.  Norovirus prevalence and estimated viral load in symptomatic and asymptomatic children from rural communities of Vhembe district, South Africa.

Authors:  Jean Pierre Kabue; Emma Meader; Paul R Hunter; Natasha Potgieter
Journal:  J Clin Virol       Date:  2016-09-13       Impact factor: 3.168

4.  Evaluation of real-time RT-PCR assays for detection and quantification of norovirus genogroups I and II.

Authors:  Kitwadee Rupprom; Porntip Chavalitshewinkoon-Petmitr; Pornphan Diraphat; Leera Kittigul
Journal:  Virol Sin       Date:  2017-02-20       Impact factor: 4.327

5.  Molecular Characterization of Norovirus Strains Isolated from Older Children and Adults in Impoverished Communities of Vhembe District, South Africa.

Authors:  G Mulondo; R Khumela; J P Kabue; A N Traore; N Potgieter
Journal:  Adv Virol       Date:  2020-06-29

6.  Evaluation of RIDA®GENE norovirus GI/GII real time RT-PCR using stool specimens collected from children and adults with acute gastroenteritis.

Authors:  N Kanwar; F Hassan; L Barclay; C Langley; J Vinjé; P W Bryant; K St George; L Mosher; J M Matthews-Greer; M A Rocha; D O Beenhouwer; C J Harrison; M Moffatt; N Shastri; R Selvarangan
Journal:  J Clin Virol       Date:  2018-04-10       Impact factor: 3.168

7.  Unveiling Viruses Associated with Gastroenteritis Using a Metagenomics Approach.

Authors:  Xavier Fernandez-Cassi; Sandra Martínez-Puchol; Marcelle Silva-Sales; Thais Cornejo; Rosa Bartolome; Silvia Bofill-Mas; Rosina Girones
Journal:  Viruses       Date:  2020-12-13       Impact factor: 5.048

Review 8.  Acute Infectious Gastroenteritis: The Causative Agents, Omics-Based Detection of Antigens and Novel Biomarkers.

Authors:  Haziqah Hasan; Nor Ashika Nasirudeen; Muhammad Alif Farhan Ruzlan; Muhammad Aiman Mohd Jamil; Noor Akmal Shareela Ismail; Asrul Abdul Wahab; Adli Ali
Journal:  Children (Basel)       Date:  2021-12-02
  8 in total

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