Literature DB >> 2476446

A novel in situ colony screening method to detect human immunodeficiency virus reverse transcriptase activity expressed in bacteria. Isolation of pseudorevertants of reverse transcriptase mutants.

V R Prasad1, S P Goff.   

Abstract

An in situ screening procedure is described that permits the detection of RNA-dependent DNA polymerase activity in bacterial colonies expressing the reverse transcriptase of the human immunodeficiency virus. The procedure has been applied to the isolation of rare pseudorevertants of an inactive parental mutant.

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Year:  1989        PMID： 2476446

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  6 in total

1.  Isolation and characterization of a dideoxyguanosine triphosphate-resistant mutant of human immunodeficiency virus reverse transcriptase.

Authors:  V R Prasad; I Lowy; T de los Santos; L Chiang; S P Goff
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-15       Impact factor: 11.205

2.  Conferring RNA polymerase activity to a DNA polymerase: a single residue in reverse transcriptase controls substrate selection.

Authors:  G Gao; M Orlova; M M Georgiadis; W A Hendrickson; S P Goff
Journal:  Proc Natl Acad Sci U S A       Date:  1997-01-21       Impact factor: 11.205

3.  Increased polymerase fidelity of E89G, a nucleoside analog-resistant variant of human immunodeficiency virus type 1 reverse transcriptase.

Authors:  W C Drosopoulos; V R Prasad
Journal:  J Virol       Date:  1996-07       Impact factor: 5.103

4.  Mutations that confer resistance to template-analog inhibitors of human immunodeficiency virus (HIV) type 1 reverse transcriptase lead to severe defects in HIV replication.

Authors:  Timothy S Fisher; Pheroze Joshi; Vinayaka R Prasad
Journal:  J Virol       Date:  2002-04       Impact factor: 5.103

5.  Mutagenesis of the Glu-89 residue in human immunodeficiency virus type 1 (HIV-1) and HIV-2 reverse transcriptases: effects on nucleoside analog resistance.

Authors:  Q Song; G Yang; S P Goff; V R Prasad
Journal:  J Virol       Date:  1992-12       Impact factor: 5.103

6.  Turnover of circulating virion RNA and of cell-associated viral DNA reflects active viral replication in human immunodeficiency virus type 1-infected individuals.

Authors:  D W Lineberger; J A Kessler; J A Waterbury; V W Byrnes; F Massari; S Staszewski; E A Emini
Journal:  J Virol       Date:  1995-04       Impact factor: 5.103
  6 in total

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