| Literature DB >> 24759738 |
Zhangqian Chen1, Lin Zhang1, Limin Xia1, Yangsheng Jin2, Qing Wu2, Hao Guo1, Xin Shang1, Jianhua Dou1, Kaichun Wu1, Yongzhan Nie3, Daiming Fan4.
Abstract
Understanding the mechanism underlying multidrug resistance and identifying effective targets that can overcome it is of critical importance. In this study, mRNA and miRNA expression profiling of the drug resistant sublines, SGC7901/VCR and SGC7901/ADR, and their parental gastric cancer cell line SGC7901 were performed. A significant number of genes and a limited subset of miRNAs were commonly dysregulated, which were further validated using qRT-PCR. GO and KEGG pathway analyses of the commonly dysregulated genes indicated that the MAPK signalling pathway may be involved in multidrug resistance, which was further validated using immunoblotting and MTT assay. Finally a primary multidrug resistance network in gastric cancer, consisting of the commonly dysregulated genes and miRNAs, was established and functional miRNA-mRNA pairs were identified. The commonly dysregulated genes and miRNAs identified in this study may represent good therapeutic targets and further study of these targets may increase our understanding of the mechanisms underlying multidrug resistance.Entities:
Keywords: Expression profiling; Gastric cancer; Mechanism; Multidrug resistance; Therapeutic target
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Year: 2014 PMID： 24759738 DOI： 10.1016/j.canlet.2014.04.010
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679