Literature DB >> 2475776

Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes.

J J Lafaille1, J Linss, M A Krieger, T Souto-Padrón, W de Souza, S Goldenberg.   

Abstract

Trypanosoma cruzi genes were cloned in lambda gt11 and screened with an anti-trypomastigote antiserum. Two out of twelve clones were selected in view of their reactivity with human chagasic sera. One clone encodes a flagellar antigen (FRA) of more than 300 kDa, whereas the other corresponds to a roughly 225-kDa cytoplasmic antigen (CRA). The flagellar antigen is present in both epimastigotes and trypomastigotes, but the cytoplasmic antigen is not found in trypomastigotes. The CRA clone is entirely composed of at least 23 copies of a 42-bp repeat and the FRA gene contains at least 14 copies of a 204-bp motif. The FRA gene hybridizes to a RNA of about 10 kb, while the CRA gene detects a transcript of 5.2 kb.

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Year:  1989        PMID： 2475776     DOI： 10.1016/0166-6851(89)90115-1

Source DB:  PubMed          Journal:  Mol Biochem Parasitol        ISSN： 0166-6851            Impact factor:   1.759


  23 in total

1.  Physical mapping of a 670-kb region of chromosomes XVI and XVII from the human protozoan parasite Trypanosoma cruzi encompassing the genes for two immunodominant antigens.

Authors:  M R Santos; H Lorenzi; P Porcile; M S Carmo; A Schijman; A Brandão; J E Araya; H B Gomes; M A Chiurillo; J L Ramirez; W M Degrave; M J Levin; J F da Silveira
Journal:  Genome Res       Date:  1999-12       Impact factor: 9.043

2.  Excretory-secretory antigens of Trypanosoma cruzi are potentially useful for serodiagnosis of chronic Chagas' disease.

Authors:  M Nakazawa; D S Rosa; V R Pereira; M O Moura; V C Furtado; W V Souza; M N Barros; F G Abath; Y M Gomes
Journal:  Clin Diagn Lab Immunol       Date:  2001-09

Review 3.  [Molecular biological techniques in the diagnosis of tropical parasitic diseases].

Authors:  R Felleisen; M Q Klinkert
Journal:  Naturwissenschaften       Date:  1992-11

4.  Comparison of recombinant Trypanosoma cruzi peptide mixtures versus multiepitope chimeric proteins as sensitizing antigens for immunodiagnosis.

Authors:  Cecilia Camussone; Verónica Gonzalez; María S Belluzo; Nazarena Pujato; María E Ribone; Claudia M Lagier; Iván S Marcipar
Journal:  Clin Vaccine Immunol       Date:  2009-04-01

5.  Serodiagnosis of Chagas' disease by enzyme-linked immunosorbent assay using two synthetic peptides as antigens.

Authors:  J M Peralta; M G Teixeira; W G Shreffler; J B Pereira; J M Burns; P R Sleath; S G Reed
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948

Review 6.  Chagas Disease Diagnostic Applications: Present Knowledge and Future Steps.

Authors:  V Balouz; F Agüero; C A Buscaglia
Journal:  Adv Parasitol       Date:  2016-11-14       Impact factor: 3.870

7.  Expression in Escherichia coli of a dominant immunogen of Trypanosoma cruzi recognized by human chagasic sera.

Authors:  P C Cotrim; G S Paranhos; R A Mortara; J Wanderley; A Rassi; M E Camargo; J F da Silveira
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

8.  Immunological dominance of Trypanosoma cruzi tandem repeat proteins.

Authors:  Yasuyuki Goto; Darrick Carter; Steven G Reed
Journal:  Infect Immun       Date:  2008-07-14       Impact factor: 3.441

9.  Identification and synthesis of a major conserved antigenic epitope of Trypanosoma cruzi.

Authors:  J M Burns; W G Shreffler; D E Rosman; P R Sleath; C J March; S G Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1992-02-15       Impact factor: 11.205

10.  A recombinant Leishmania chagasi antigen that stimulates cellular immune responses in infected mice.

Authors:  M E Wilson; B M Young; K P Andersen; J V Weinstock; A Metwali; K M Ali; J E Donelson
Journal:  Infect Immun       Date:  1995-05       Impact factor: 3.441
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