Zhang-Hua Li1, Wen Liao2, Qiang Zhao3, Tang Huan1, Pan Feng1, Xia Wei3, Yang Yi4, Ning-Sheng Shao3. 1. Department of Orthopedics, Renmin Hospital of Wuhan University Wuhan 430060, China. 2. Hubei University of Chinese Medicine Wuhan 430065, China. 3. Laboratory of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences Beijing 100850, China. 4. Wuhan Sports University Wuhan 430079, China.
Abstract
OBJECTIVE: To observe the effect of Cbfa1 on biological characteristics of marrow mesenchymal stem cells under hypoxia. METHODS: The second passage of the MSCs were transfected with Cbfa1 and then cultured in 20% O2 and 3% O2 condition individually. The biological features of the cultured MSCs were assessed by the Real-time PCR. RESULTS: After transfected with Cbfa1, the morphology of MSCs was no significant difference between two oxygen concentrations; The RT-PCR examination revealed that the expression of Cbfa1, BMP, OPN and VEGF in MSCs was higher than those before Cbfa1 transfection (P<0.05), especial the expression of Cbfa1 (P<0.05). CONCLUSION: After transfected with Cbfa1, cell morphology or growth cycle of MSCs was not significantly affected, but its osteogenic differentiation potential enhanced, particularly its osteogenic differentiation potential under hypoxia enhanced.
OBJECTIVE: To observe the effect of Cbfa1 on biological characteristics of marrow mesenchymal stem cells under hypoxia. METHODS: The second passage of the MSCs were transfected with Cbfa1 and then cultured in 20% O2 and 3% O2 condition individually. The biological features of the cultured MSCs were assessed by the Real-time PCR. RESULTS: After transfected with Cbfa1, the morphology of MSCs was no significant difference between two oxygen concentrations; The RT-PCR examination revealed that the expression of Cbfa1, BMP, OPN and VEGF in MSCs was higher than those before Cbfa1 transfection (P<0.05), especial the expression of Cbfa1 (P<0.05). CONCLUSION: After transfected with Cbfa1, cell morphology or growth cycle of MSCs was not significantly affected, but its osteogenic differentiation potential enhanced, particularly its osteogenic differentiation potential under hypoxia enhanced.
Authors: Beatriz Ranera; Ana Rosa Remacha; Samuel Álvarez-Arguedas; Antonio Romero; Francisco José Vázquez; Pilar Zaragoza; Inmaculada Martín-Burriel; Clementina Rodellar Journal: BMC Vet Res Date: 2012-08-22 Impact factor: 2.741
Authors: Akram Mirzaei; Saeed Mohammadi; Seyed H Ghaffari; Marjan Yaghmaie; Mohammad Vaezi; Kamran Alimoghaddam; Ardeshir Ghavamzadeh Journal: Asian Pac J Cancer Prev Date: 2018-03-27