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Literature DB >> 2474389

A micro-method for measuring total protein in cerebrospinal fluid by using benzethonium chloride in microtiter plate wells.

R W Luxton¹, P Patel, G Keir, E J Thompson.

Abstract

By using a benzethonium chloride concentration 12-fold that described originally (Clin Chem 1979;25:1317-9), we developed a reliable method suitable for routine measurement of total protein in cerebrospinal fluid. Only 10 microL of sample is required. Reactivity to immunoglobulin G (IgG) and to albumin (Alb) is similar, as is necessary for specimens that can have very varied IgG/Alb ratios. The assay, performed in a microtiter plate for ease of use, has a between-batch coefficient of variation of 3.4% for a protein concentration of 450 mg/L. This contrasts with a dye-binding technique with Ponceau S, for which the CV was 9% at the same concentration.

Entities: Chemical Disease Gene

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Year: 1989 PMID： 2474389

Source DB: PubMed Journal: Clin Chem ISSN： 0009-9147 Impact factor: 8.327

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Cited

11 in total

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A micro-method for measuring total protein in cerebrospinal fluid by using benzethonium chloride in microtiter plate wells.

1. Surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS): a new proteomic urinary test for patients with urolithiasis.

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4. Oligoclonal banding of IgG in CSF, blood-brain barrier function, and MRI findings in patients with sarcoidosis, systemic lupus erythematosus, and Behçet's disease involving the nervous system.

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