Biochemical properties of isolated transverse tubular membranes.

This review addresses the major biochemical and structural characteristics of isolated transverse tubule (T-tubule) membranes, including methods of isolation and morphology of purified membranes, evaluation of attendant membrane activities, including ion pumps and channels, and structural and compositional analyses of functionally relevant components. Particular emphasis is placed on the Mg2+-ATPase, its localization in the T-system, its unusual kinetic properties, its possible functions, and its potential regulation by diacylglycerol and other biologically-relevant lipids. Conclusions are drawn with respect to the biochemical markers characteristic of T-tubule membranes and the criteria to be applied in the assessment of isolated T-tubule membrane purity.