Literature DB >> 2471093

Analysis of brain and myelin lipids by high-performance thin layer chromatography and densitometry.

A Di Biase1, S Salvati, G S Crescenzi.   

Abstract

We have developed a simple method involving high-performance thin layer chromatographic separation of total brain and myelin lipids. Only two solvent systems consisting of chloroform: methanol:acetic acid and water at different concentrations were needed. The plate was then stained with three sequential procedures to visualize phospholipids, cholesterol and galactolipids. Densitometric procedure at each step of staining was utilized to obtain quantitative analysis of brain and myelin samples.

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Year:  1989        PMID: 2471093     DOI: 10.1007/bf00969631

Source DB:  PubMed          Journal:  Neurochem Res        ISSN: 0364-3190            Impact factor:   3.996


  13 in total

1.  A simple method for the isolation and purification of total lipides from animal tissues.

Authors:  J FOLCH; M LEES; G H SLOANE STANLEY
Journal:  J Biol Chem       Date:  1957-05       Impact factor: 5.157

2.  The quantitative estimation of cerebrosides in nervous tissue.

Authors:  L SVENNERHOLM
Journal:  J Neurochem       Date:  1956-05       Impact factor: 5.372

3.  Microdetermination of lipid classes after thin-layer chromatography.

Authors:  J J Kabara; J S Chen
Journal:  Anal Chem       Date:  1976-05       Impact factor: 6.986

Review 4.  Quantitative analysis of lipid classes.

Authors:  O S Privett; K A Dougherty; J D Castell
Journal:  Am J Clin Nutr       Date:  1971-10       Impact factor: 7.045

5.  Myelination in rat brain: changes in myelin composition during brain maturation.

Authors:  W T Norton; S E Poduslo
Journal:  J Neurochem       Date:  1973-10       Impact factor: 5.372

6.  Biochemical maturation of the central nervous system. I. Lipid changes.

Authors:  K B Dalal; E R Einstein
Journal:  Brain Res       Date:  1969-12       Impact factor: 3.252

7.  Nano-scale densitometric quantitation of phospholipids.

Authors:  E Hedegaard; B Jensen
Journal:  J Chromatogr       Date:  1981-10-09

8.  Separation of serum high-density lipoprotein for cholesterol determination: ultracentrifugation vs precipitation with sodium phosphotungstate and magnesium chloride.

Authors:  L Seigler; W T Wu
Journal:  Clin Chem       Date:  1981-06       Impact factor: 8.327

9.  Unidimensional thin-layer chromatography of phospholipids on boric acid-impregnated plates.

Authors:  J B Fine; H Sprecher
Journal:  J Lipid Res       Date:  1982-05       Impact factor: 5.922

10.  Microanalysis of complex tissue lipids by high-performance thin-layer chromatography.

Authors:  J K Yao; G M Rastetter
Journal:  Anal Biochem       Date:  1985-10       Impact factor: 3.365

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  7 in total

1.  Effects of exogenous hexacosanoic acid on biochemical myelin composition in weaning and post-weaning rats.

Authors:  A Di Biase; C Avellino; F Pieroni; T Quaresima; A Grisolia; M Cappa; S Salvati
Journal:  Neurochem Res       Date:  1997-03       Impact factor: 3.996

2.  Influences of hypothyroidism on lipid composition and inositol lipid-linked receptors responsiveness and protein kinase C (PKC) activity in the cerebral cortex of Lewis rats.

Authors:  A Pintor; S Fortuna; P Lorenzini; A Pascale; F Battaini; C Avellino; L Malvezzi Campeggi; S Salvati
Journal:  Neurochem Res       Date:  1996-05       Impact factor: 3.996

3.  Sulfatides Primarily Exist in the Substantia Nigra Region of Mouse Brain Tissue.

Authors:  Kab-Tae Park; Jong Cheol Shon; Ji-Eun Kim; Gyu Hwan Park; Hyun Jin Choi; Kwang-Hyeon Liu
Journal:  Lipids       Date:  2017-01-11       Impact factor: 1.880

4.  Effect of propylthiouracil-induced hypothyroidism on cerebral cortex of young and aged rats: lipid composition of synaptosomes, muscarinic receptor sites, and acetylcholinesterase activity.

Authors:  S Salvati; L Attorri; L M Campeggi; A Olivieri; M Sorcini; S Fortuna; A Pintor
Journal:  Neurochem Res       Date:  1994-09       Impact factor: 3.996

5.  Lipid changes in central nervous system membranes in experimental allergic encephalomyelitis (EAE).

Authors:  S Salvati; L Attorri; A Confaloni; A Di Biase
Journal:  Neurochem Res       Date:  1990-11       Impact factor: 3.996

6.  Effect of propylthiouracil-induced hypothyroidism on membranes of adult rat brain.

Authors:  S Salvati; L M Campeggi; M Sorcini; A Olivieri; A Di Biase
Journal:  Lipids       Date:  1993-12       Impact factor: 1.880

7.  Lipid profile of rat myelin subfractions.

Authors:  A Di Biase; S Salvati; G Serlupi Crescenzi
Journal:  Neurochem Res       Date:  1990-05       Impact factor: 3.996

  7 in total

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