Literature DB >> 24710612

Impact of short-time urine freezing on the sensitivity of an established Schistosoma real-time PCR assay.

Hilaire M Kenguele1, Ayola A Adegnika2, Anne-Marie Nkoma2, Ulysse Ateba-Ngoa2, Mirabeau Mbong2, Jeannot Zinsou2, Bertrand Lell2, Jaco J Verweij2.   

Abstract

Urogenital schistosomiaisis is a serious public health problem in sub-Saharan Africa. In this study, we have updated an established real-time polymerase chain reaction (PCR) routinely used in our laboratory. Schistosoma genus-specific real-time PCR was performed on DNA isolated from 85 urine samples and pellets obtained after centrifugation without and after frozen storage. The results revealed that concentration by centrifugation of the urine samples and freezing of the samples before extracting DNA improves the sensitivity of the PCR. © The American Society of Tropical Medicine and Hygiene.

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Year:  2014        PMID： 24710612      PMCID： PMC4047745          DOI： 10.4269/ajtmh.14-0005

Source DB:  PubMed          Journal:  Am J Trop Med Hyg        ISSN： 0002-9637            Impact factor:   2.345


  17 in total

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