H-S Xu1, H-L Zong, M Shang, X Ming, J-P Zhao, C Ma, L Cao. 1. Department of Neurosurgery, the Central Hospital of Xuzhou, Xuzhou Clinical School of Xuzhou Medical College, Affiliated Hospital of Southeast University, Xuzhou, Jiangsu, People's Republic of China. xhs51@126.com.
Abstract
OBJECTIVES: To study the effects of the miR-324-5p on the glioma cells proliferation via the targeted regulation of the glioma-associated oncogene 1. METHODS: The luciferase reporter gene was used to test whether the glioma-associated oncogene 1 was the target of the miR-324-5p microRNA. The glioma-associated oncogene 1 expression was detected by Western blot. The proliferation and cell cycle were evaluated by MTT assay and flow cytometry. RESULTS: The glioma-associated oncogene 1 is a target of the miR-324-5p. An over-expressed miR-324-5p could reduce the cell survival rate and increase the G1/G0 phase rate in the glioma cell lines. CONCLUSIONS: The miR-324-5p can inhibit proliferation of the glioma cells via the targeted regulation of the glioma-associated oncogene 1.
OBJECTIVES: To study the effects of the miR-324-5p on the glioma cells proliferation via the targeted regulation of the glioma-associated oncogene 1. METHODS: The luciferase reporter gene was used to test whether the glioma-associated oncogene 1 was the target of the miR-324-5p microRNA. The glioma-associated oncogene 1 expression was detected by Western blot. The proliferation and cell cycle were evaluated by MTT assay and flow cytometry. RESULTS: The glioma-associated oncogene 1 is a target of the miR-324-5p. An over-expressed miR-324-5p could reduce the cell survival rate and increase the G1/G0 phase rate in the glioma cell lines. CONCLUSIONS: The miR-324-5p can inhibit proliferation of the glioma cells via the targeted regulation of the glioma-associated oncogene 1.
Authors: Durgesh Tiwari; Darrin H Brager; Jeffrey K Rymer; Alexander T Bunk; Angela R White; Nada A Elsayed; Joseph C Krzeski; Andrew Snider; Lindsay M Schroeder Carter; Steve C Danzer; Christina Gross Journal: Neurobiol Dis Date: 2019-06-15 Impact factor: 5.996
Authors: Krzysztof Zakrzewski; Michał Jarząb; Aleksandra Pfeifer; Małgorzata Oczko-Wojciechowska; Barbara Jarząb; Paweł P Liberski; Magdalena Zakrzewska Journal: BMC Cancer Date: 2015-10-24 Impact factor: 4.430