Literature DB >> 2470151

Stereochemical course of catalysis by the Tetrahymena ribozyme.

J Rajagopal1, J A Doudna, J W Szostak.   

Abstract

The group I intron from Tetrahymena catalyzes phosphodiester transfer reactions on various RNA substrates. A modified RNA substrate with a phosphorothioate group in one stereoisomeric form at the site of reaction was synthesized in order to determine the stereochemical course of an RNA-catalyzed reaction. The reaction product was digested with a stereospecific nuclease to determine the configuration of the product phosphorothioate. The reaction occurs with inversion of configuration at phosphorus, implying an in-line pathway for the reaction.

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Year:  1989        PMID： 2470151     DOI： 10.1126/science.2470151

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  23 in total

Review 1.  Recent advances in the elucidation of the mechanisms of action of ribozymes.

Authors:  Y Takagi; M Warashina; W J Stec; K Yoshinari; K Taira
Journal:  Nucleic Acids Res       Date:  2001-05-01       Impact factor: 16.971

Review 2.  Biological phosphoryl-transfer reactions: understanding mechanism and catalysis.

Authors:  Jonathan K Lassila; Jesse G Zalatan; Daniel Herschlag
Journal:  Annu Rev Biochem       Date:  2011       Impact factor: 23.643

3.  Dissection of a metal-ion-mediated conformational change in Tetrahymena ribozyme catalysis.

Authors:  Shu-ou Shan; Daniel Herschlag
Journal:  RNA       Date:  2002-07       Impact factor: 4.942

4.  Biography of Jennifer A. Doudna.

Authors:  Melissa Marino
Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-01       Impact factor: 11.205

5.  Phosphorothioate substitution identifies phosphate groups important for pre-mRNA splicing.

Authors:  K L Maschhoff; R A Padgett
Journal:  Nucleic Acids Res       Date:  1992-04-25       Impact factor: 16.971

6.  The chemical basis of adenosine conservation throughout the Tetrahymena ribozyme.

Authors:  L Ortoleva-Donnelly; A A Szewczak; R R Gutell; S A Strobel
Journal:  RNA       Date:  1998-05       Impact factor: 4.942

7.  The methylation of one specific guanosine in a pre-tRNA prevents cleavage by RNase P and by the catalytic M1 RNA.

Authors:  D Kahle; U Wehmeyer; S Char; G Krupp
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971

8.  Uridine insertion into preedited mRNA by a mitochondrial extract from Leishmania tarentolae: stereochemical evidence for the enzyme cascade model.

Authors:  G C Frech; L Simpson
Journal:  Mol Cell Biol       Date:  1996-08       Impact factor: 4.272

9.  Metal ion interaction with cosubstrate in self-splicing of group I introns.

Authors:  A S Sjögren; E Pettersson; B M Sjöberg; R Strömberg
Journal:  Nucleic Acids Res       Date:  1997-02-01       Impact factor: 16.971

10.  The two steps of group II intron self-splicing are mechanistically distinguishable.

Authors:  M Podar; P S Perlman; R A Padgett
Journal:  RNA       Date:  1998-08       Impact factor: 4.942
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