Wei Ma1, Kai-Jin Wang2, Chen-Shu Cheng2, Guo-qing Yan2, Wei-Li Lu1, Jing-Fang Ge1, Yong-Xian Cheng3, Ning Li4. 1. Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei 230032, PR China. 2. School of Life Sciences, Anhui University, 3 Feixi Road, Hefei 230039, PR China. 3. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China. Electronic address: yxcheng@mail.kib.ac.cn. 4. Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei 230032, PR China. Electronic address: ln0110@sina.com.
Abstract
ETHNOPARMACOLOGICAL RELEVANCE: The fruit of Cornus officinalis, called "Shanzhuyu", a traditional medicine in China, is used for the treatment of kidney diseases, including diabetic nephropathy. The aim of this study is to investigate the anti-diabetic nephropathy activity of Shanzhuyu and the active compounds in the fruit. MATERIALS AND METHODS: The air dried fruit of Cornus officinalis was extracted in 80% EtOH, the obtained residue was fractioned on D101 resin column eluted with H2O/EtOH solution to get five crude fractions (fr. A-E). The anti-diabetic nephropathy activity of fractions (fr. A-E) was evaluated in vitro by inhibiting the expression of collagen IV (Col V), fibronectin (FN) and IL-6 in high-glucose-induced mesangial cells. By preliminary bio-assay screenings, repeated column chromatography on fraction B-D led the isolation of 22 compounds, whose structures were determined by extensive spectroscopic analysis, and the anti-diabetic nephropathy activity of the isolated compounds was also evaluated. RESULTS: Two new iridoid glucosides, logmalicids A and B (1 and 2), together with 20 known compounds (3-22) were isolated from the extract of Shanzhuyu under the bioassay-guided screenings. The anti-diabetic nephropathy activity assay displayed that fractions A, D and E could significantly inhibit the production of Col IV; fractions A and C could significantly inhibit the expression of FN and IL-6 in the high-glucose-stimulated mesangial cells at concentration of 50 μg/mL; and loganin (3) and its derivatives (1 and 2) could significantly inhibit the expression of FN and IL-6 at concentration of 10 μM, respectively. CONCLUSIONS: The results suggested that loganin and its derivatives were the active compounds in Cornus officinalis fruit (Shanzhuyu) on diabetic nephropathy. This study further supported the traditional use of Shanzhuyu to treat diabetic nephropathy and related kidney diseases.
ETHNOPARMACOLOGICAL RELEVANCE: The fruit of Cornus officinalis, called "Shanzhuyu", a traditional medicine in China, is used for the treatment of kidney diseases, including diabetic nephropathy. The aim of this study is to investigate the anti-diabetic nephropathy activity of Shanzhuyu and the active compounds in the fruit. MATERIALS AND METHODS: The air dried fruit of Cornus officinalis was extracted in 80% EtOH, the obtained residue was fractioned on D101 resin column eluted with H2O/EtOH solution to get five crude fractions (fr. A-E). The anti-diabetic nephropathy activity of fractions (fr. A-E) was evaluated in vitro by inhibiting the expression of collagen IV (Col V), fibronectin (FN) and IL-6 in high-glucose-induced mesangial cells. By preliminary bio-assay screenings, repeated column chromatography on fraction B-D led the isolation of 22 compounds, whose structures were determined by extensive spectroscopic analysis, and the anti-diabetic nephropathy activity of the isolated compounds was also evaluated. RESULTS: Two new iridoidglucosides, logmalicids A and B (1 and 2), together with 20 known compounds (3-22) were isolated from the extract of Shanzhuyu under the bioassay-guided screenings. The anti-diabetic nephropathy activity assay displayed that fractions A, D and E could significantly inhibit the production of Col IV; fractions A and C could significantly inhibit the expression of FN and IL-6 in the high-glucose-stimulated mesangial cells at concentration of 50 μg/mL; and loganin (3) and its derivatives (1 and 2) could significantly inhibit the expression of FN and IL-6 at concentration of 10 μM, respectively. CONCLUSIONS: The results suggested that loganin and its derivatives were the active compounds in Cornus officinalis fruit (Shanzhuyu) on diabetic nephropathy. This study further supported the traditional use of Shanzhuyu to treat diabetic nephropathy and related kidney diseases.
Authors: Dorota Szumny; Tomasz Sozański; Alicja Z Kucharska; Wojciech Dziewiszek; Narcyz Piórecki; Jan Magdalan; Ewa Chlebda-Sieragowska; Robert Kupczynski; Adam Szeląg; Antoni Szumny Journal: Evid Based Complement Alternat Med Date: 2015-06-01 Impact factor: 2.629