Literature DB >> 24685427

Relaxed tRNA specificity of the Staphylococcus aureus aspartyl-tRNA synthetase enables RNA-dependent asparagine biosynthesis.

Stefani R Mladenova1, Kathryn R Stein1, Lianne Bartlett1, Kelly Sheppard2.   

Abstract

The human pathogen Staphylococcus aureus is an asparagine prototroph despite its genome not encoding an asparagine synthetase. S. aureus does use an asparaginyl-tRNA synthetase (AsnRS) to directly ligate asparagine to tRNA(Asn). The S. aureus genome also codes for one aspartyl-tRNA synthetase (AspRS). Here we demonstrate the lone S. aureus aspartyl-tRNA synthetase has relaxed tRNA specificity and can be used with the amidotransferase GatCAB to synthesize asparagine on tRNA(Asn). S. aureus thus encodes both the direct and indirect routes for Asn-tRNA(Asn) formation while encoding only one aspartyl-tRNA synthetase. The presence of the indirect pathway explains how S. aureus synthesizes asparagine without either asparagine synthetase.
Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Asparagine biosynthesis; Aspartyl-tRNA synthetase; RNA-dependent; Staphylococcus aureus; tRNA

Mesh：

Substances：

Year:  2014        PMID： 24685427     DOI： 10.1016/j.febslet.2014.03.042

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  4 in total

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Journal:  PLoS One       Date:  2014-10-22       Impact factor: 3.240

3.  Bacterial Aspartyl-tRNA Synthetase Has Glutamyl-tRNA Synthetase Activity.

Authors:  Udumbara M Rathnayake; Tamara L Hendrickson
Journal:  Genes (Basel)       Date:  2019-04-01       Impact factor: 4.096

4.  A computational knowledge-base elucidates the response of Staphylococcus aureus to different media types.

Authors:  Yara Seif; Jonathan M Monk; Nathan Mih; Hannah Tsunemoto; Saugat Poudel; Cristal Zuniga; Jared Broddrick; Karsten Zengler; Bernhard O Palsson
Journal:  PLoS Comput Biol       Date:  2019-01-09       Impact factor: 4.475
  4 in total

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