Literature DB >> 24677814

Characterization of individual mouse cerebrospinal fluid proteomes.

Jeffrey S Smith1, Thomas E Angel, Charles Chavkin, Daniel J Orton, Ronald J Moore, Richard D Smith.   

Abstract

Analysis of cerebrospinal fluid (CSF) offers key insight into the status of the CNS. Characterization of murine CSF proteomes can provide a valuable resource for studying CNS injury and disease in animal models. However, the small volume of CSF in mice has thus far limited individual mouse proteome characterization. Through nonterminal CSF extractions in C57Bl/6 mice and high-resolution 2D-LC MS/MS analysis of individual murine samples, we report the most comprehensive proteome characterization of individual murine CSF to date. We identified a total of 566 unique proteins, including 128 proteins from three individual CSF samples that have been previously identified in brain tissue. Our methods and analysis provide a mechanism for individual murine CSF proteome analysis. The data are available in the ProteomeXchange with identifier PXD000248 (http://proteomecentral.proteomexchange.org/dataset/PXD000248).
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  Animal proteomics; Cerebrospinal fluid; MS; Mouse; Proteome

Mesh:

Substances:

Year:  2014        PMID: 24677814      PMCID: PMC4044923          DOI: 10.1002/pmic.201300241

Source DB:  PubMed          Journal:  Proteomics        ISSN: 1615-9853            Impact factor:   3.984


  24 in total

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