BACKGROUND: Hypoxia, metabolism, and growth factor signaling are important prognostic features in most solid tumors. The purpose of this study was to determine whether head and neck squamous cell carcinoma (HNSCC) xenografts show similar biological and molecular characteristics as the primary tumor they originate from. METHODS: Eighteen HNSCC primary tumor-xenograft pairs were immunofluorescently stained for pimonidazole (hypoxia), carbonic anhydrase IX (CAIX), glucose transporter-1 (GLUT-1), monocarboxylate transporter-1 (MCT-1), monocarboxylate transporter-4 (MCT-4), epidermal growth factor receptor (EGFR), and phosphorylated protein kinase B (pAKT). RESULTS: Although no correlation was found for the amount of hypoxia, significant correlations between primary tumors and xenografts were observed for both the percentage of cells positive for expression and the hypoxia-related expression pattern of CAIX, GLUT-1, and MCT-1. For EGFR and MCT-4, the intensity of expression was correlated. No correlation was observed for pAKT. CONCLUSION: Xenografts did not always resemble the primary tumor they originate from, but the xenografts did represent the variability in expression levels and patterns observed in the primary tumors.
BACKGROUND:Hypoxia, metabolism, and growth factor signaling are important prognostic features in most solid tumors. The purpose of this study was to determine whether head and neck squamous cell carcinoma (HNSCC) xenografts show similar biological and molecular characteristics as the primary tumor they originate from. METHODS: Eighteen HNSCC primary tumor-xenograft pairs were immunofluorescently stained for pimonidazole (hypoxia), carbonic anhydrase IX (CAIX), glucose transporter-1 (GLUT-1), monocarboxylate transporter-1 (MCT-1), monocarboxylate transporter-4 (MCT-4), epidermal growth factor receptor (EGFR), and phosphorylated protein kinase B (pAKT). RESULTS: Although no correlation was found for the amount of hypoxia, significant correlations between primary tumors and xenografts were observed for both the percentage of cells positive for expression and the hypoxia-related expression pattern of CAIX, GLUT-1, and MCT-1. For EGFR and MCT-4, the intensity of expression was correlated. No correlation was observed for pAKT. CONCLUSION: Xenografts did not always resemble the primary tumor they originate from, but the xenografts did represent the variability in expression levels and patterns observed in the primary tumors.
Authors: Casper Reijnen; Willem Jan van Weelden; Martijn S J P Arts; Johan P Peters; Paul F Rijken; Koen van de Vijver; Maria Santacana; Peter Bronsert; Johan Bulten; Marc Hirschfeld; Eva Colas; Antonio Gil-Moreno; Armando Reques; Gemma Mancebo; Camilla Krakstad; Jone Trovik; Ingfrid S Haldorsen; Jutta Huvila; Martin Koskas; Vit Weinberger; Lubos Minar; Eva Jandakova; Marc P L M Snijders; Saskia van den Berg-van Erp; Heidi V N Küsters-Vandevelde; Xavier Matias-Guiu; Frederic Amant; Leon F A G Massuger; Johan Bussink; Johanna M A Pijnenborg Journal: Br J Cancer Date: 2019-04-23 Impact factor: 7.640
Authors: Tet Woo Lee; Amy Lai; Julia K Harms; Dean C Singleton; Benjamin D Dickson; Andrew M J Macann; Michael P Hay; Stephen M F Jamieson Journal: Cancers (Basel) Date: 2020-12-12 Impact factor: 6.639
Authors: Bianca A W Hoeben; Maud H W Starmans; Ralph T H Leijenaar; Ludwig J Dubois; Albert J van der Kogel; Johannes H A M Kaanders; Paul C Boutros; Philippe Lambin; Johan Bussink Journal: BMC Cancer Date: 2014-02-26 Impact factor: 4.430