Recent developments in the field of bending rigidity measurements on membranes.

This review gives a brief overview of experimental approaches used to assess the bending rigidity of membranes. Emphasis is placed on techniques based on the use of giant unilamellar vesicles. We summarize the effect on the bending rigidity of membranes as a function of membrane composition, presence of various inclusions in the bilayer and molecules and ions in the bathing solutions. Examples for the impact of temperature, cholesterol, some peptides and proteins, sugars and salts are provided and the literature data are discussed critically. Future directions, open questions and possible developments in this research field are also included.