Literature DB >> 24659822

Creation and Evaluation of a Single-chain Antibody Tetramer that Targets Brain Endothelial Cells.

Xiaobin Zhang1, Xin Xiang Wang1, Eric V Shusta1.   

Abstract

Antibodies that target and internalize into blood-brain barrier (BBB) endothelial cells offer promise as drug delivery agents. Previously, we identified a single-chain antibody (scFvA) capable of binding to the BBB. In an attempt to improve the binding and internalization properties of the single chain antibody (scFvA), a biotinylation tag (Avitag) was fused to scFvA and the protein secreted by yeast. The scFvA-Avitag could be biotinylated by yeast-displayed BirA enzyme and biotinylated scFvA-Avitag could be used to create scFv tetramers. Tetramerization of scFvA improved the internalization of scFvA into BBB endothelial cells, and biotinylated scFvA-Avitag could also be used to target streptavidin-coated quantum dots for BBB endothelial cell internalization. Perfusing the rat brain with scFvA-tetramer confirmed that the antigen targeted by scFvA is distributed on blood side of the BBB, suggesting the potential for downstream application of scFvA in brain-targeted drug delivery.

Entities:  

Year:  2014        PMID： 24659822      PMCID： PMC3958949          DOI： 10.1002/aic.14348

Source DB:  PubMed          Journal:  AIChE J        ISSN： 0001-1541            Impact factor:   3.993


  37 in total

1.  ScFv multimers of the anti-neuraminidase antibody NC10: shortening of the linker in single-chain Fv fragment assembled in V(L) to V(H) orientation drives the formation of dimers, trimers, tetramers and higher molecular mass multimers.

Authors:  O Dolezal; L A Pearce; L J Lawrence; A J McCoy; P J Hudson; A A Kortt
Journal:  Protein Eng       Date:  2000-08

2.  Streptavidin-biotin crosslinking of therapeutic enzymes with carrier antibodies: nanoconjugates for protection against endothelial oxidative stress.

Authors:  Vladimir V Shuvaev; Thomas Dziubla; Rainer Wiewrodt; Vladimir R Muzykantov
Journal:  Methods Mol Biol       Date:  2004

3.  In vivo biotinylation of the major histocompatibility complex (MHC) class II/peptide complex by coexpression of BirA enzyme for the generation of MHC class II/tetramers.

Authors:  Junbao Yang; Andrés Jaramillo; Ruili Shi; William W Kwok; T Mohanakumar
Journal:  Hum Immunol       Date:  2004-07       Impact factor: 2.850

4.  A novel method for producing mono-biotinylated, biologically active neurotrophic factors: an essential reagent for single molecule study of axonal transport.

Authors:  Kijung Sung; Michael T Maloney; Jingkun Yang; Chengbiao Wu
Journal:  J Neurosci Methods       Date:  2011-07-02       Impact factor: 2.390

5.  Mining a yeast library for brain endothelial cell-binding antibodies.

Authors:  Xin Xiang Wang; Yong Ku Cho; Eric V Shusta
Journal:  Nat Methods       Date:  2007-01-07       Impact factor: 28.547

6.  Secretion-and-capture cell-surface display for selection of target-binding proteins.

Authors:  J A Rakestraw; D Aird; P M Aha; B M Baynes; D Lipovsek
Journal:  Protein Eng Des Sel       Date:  2011-03-14       Impact factor: 1.650

7.  A versatile drug delivery system using streptavidin-tagged pegylated liposomes and biotinylated biomaterials.

Authors:  Ming-Han Chen; Yasushi Soda; Kiyoko Izawa; Seiichiro Kobayashi; Kenzaburo Tani; Kazuo Maruyama; Arinobu Tojo; Shigetaka Asano
Journal:  Int J Pharm       Date:  2013-06-24       Impact factor: 5.875

8.  Chemical modification of amine groups on PS II protein(s) retards photoassembly of the photosynthetic water-oxidizing complex.

Authors:  Yasuo Ishikawa; Yasusi Yamamoto; Mayuko Otsubo; Steven M Theg; Noriaki Tamura
Journal:  Biochemistry       Date:  2002-02-12       Impact factor: 3.162

9.  Ex vivo characterization and isolation of rare memory B cells with antigen tetramers.

Authors:  Bettina Franz; Kenneth F May; Glenn Dranoff; Kai Wucherpfennig
Journal:  Blood       Date:  2011-05-06       Impact factor: 22.113

10.  Differential binding of the lectins Griffonia simplicifolia I and Lycopersicon esculentum to microvascular endothelium: organ-specific localization and partial glycoprotein characterization.

Authors:  G A Porter; G E Palade; A J Milici
Journal:  Eur J Cell Biol       Date:  1990-02       Impact factor: 4.492
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  3 in total

1.  Site-Specific Antibody Functionalization Using Tetrazine-Styrene Cycloaddition.

Authors:  Benjamin J Umlauf; Kalie A Mix; Vanessa A Grosskopf; Ronald T Raines; Eric V Shusta
Journal:  Bioconjug Chem       Date:  2018-05-03       Impact factor: 4.774

2.  Drug Delivery Strategies to Overcome the Blood-Brain Barrier (BBB).

Authors:  Hans Christian Cederberg Helms; Mie Kristensen; Lasse Saaby; Gert Fricker; Birger Brodin
Journal:  Handb Exp Pharmacol       Date:  2022

3.  Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling.

Authors:  Dawson Fogen; Sau-Ching Wu; Kenneth Kai-Sing Ng; Sui-Lam Wong
Journal:  PLoS One       Date:  2015-09-25       Impact factor: 3.240
  3 in total

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