Literature DB >> 17206151

Mining a yeast library for brain endothelial cell-binding antibodies.

Xin Xiang Wang1, Yong Ku Cho, Eric V Shusta.   

Abstract

We describe the use of yeast surface display for the identification of antibodies that bind the plasma membranes of living cells. Yeast panning with a nonimmune human single-chain antibody library identified 34 unique lead antibodies that bind (K(d) = 82 +/- 15 nM) and in some cases internalize into rat brain endothelial cells. In addition, we used a new yeast display immunoprecipitation procedure for initial characterization of the cognate antigens.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 17206151      PMCID： PMC2637222          DOI： 10.1038/nmeth993

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  15 in total

1.  Identification of a human anti-CD55 single-chain Fv by subtractive panning of a phage library using tumor and nontumor cell lines.

Authors:  J B Ridgway; E Ng; J A Kern; J Lee; J Brush; A Goddard; P Carter
Journal:  Cancer Res       Date:  1999-06-01       Impact factor: 12.701

2.  Selection of tumor-specific internalizing human antibodies from phage libraries.

Authors:  M A Poul; B Becerril; U B Nielsen; P Morisson; J D Marks
Journal:  J Mol Biol       Date:  2000-09-01       Impact factor: 5.469

3.  Selection-dominant and nonaccessible epitopes on cell-surface receptors revealed by cell-panning with a large phage antibody library.

Authors:  H R Hoogenboom; J T Lutgerink; M M Pelsers; M J Rousch; J Coote; N Van Neer; A De Bruïne; F A Van Nieuwenhoven; J F Glatz; J W Arends
Journal:  Eur J Biochem       Date:  1999-03

4.  Isolation of human prostate cancer cell reactive antibodies using phage display technology.

Authors:  Mikhail Popkov; Christoph Rader; Carlos F Barbas
Journal:  J Immunol Methods       Date:  2004-08       Impact factor: 2.303

5.  Anti-transferrin receptor antibody and antibody-drug conjugates cross the blood-brain barrier.

Authors:  P M Friden; L R Walus; G F Musso; M A Taylor; B Malfroy; R M Starzyk
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-01       Impact factor: 11.205

Review 6.  Selecting and screening recombinant antibody libraries.

Authors:  Hennie R Hoogenboom
Journal:  Nat Biotechnol       Date:  2005-09       Impact factor: 54.908

Review 7.  The blood-brain barrier: bottleneck in brain drug development.

Authors:  William M Pardridge
Journal:  NeuroRx       Date:  2005-01

8.  Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments.

Authors:  E V Shusta; R T Raines; A Plückthun; K D Wittrup
Journal:  Nat Biotechnol       Date:  1998-08       Impact factor: 54.908

9.  Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library.

Authors:  Michael J Feldhaus; Robert W Siegel; Lee K Opresko; James R Coleman; Jane M Weaver Feldhaus; Yik A Yeung; Jennifer R Cochran; Peter Heinzelman; David Colby; Jeffrey Swers; Christilyn Graff; H Steven Wiley; K Dane Wittrup
Journal:  Nat Biotechnol       Date:  2003-01-21       Impact factor: 54.908

10.  Regulation of gamma-glutamyl transpeptidase and alkaline phosphatase activities in immortalized rat brain microvessel endothelial cells.

Authors:  F Roux; O Durieu-Trautmann; N Chaverot; M Claire; P Mailly; J M Bourre; A D Strosberg; P O Couraud
Journal:  J Cell Physiol       Date:  1994-04       Impact factor: 6.384
View more
  43 in total

1.  A yeast display immunoprecipitation screen for targeted discovery of antibodies against membrane protein complexes.

Authors:  Jason M Lajoie; Yong Ku Cho; Dustin Frost; Samantha Bremner; Lingjun Li; Eric V Shusta
Journal:  Protein Eng Des Sel       Date:  2019-12-31       Impact factor: 1.650

Review 2.  Blood-brain barrier transport of therapeutics via receptor-mediation.

Authors:  Angela R Jones; Eric V Shusta
Journal:  Pharm Res       Date:  2007-07-10       Impact factor: 4.200

3.  Efficient recovery of high-affinity antibodies from a single-chain Fab yeast display library.

Authors:  Laura M Walker; Diana R Bowley; Dennis R Burton
Journal:  J Mol Biol       Date:  2009-04-16       Impact factor: 5.469

4.  Isolation of αL I domain mutants mediating firm cell adhesion using a novel flow-based sorting method.

Authors:  Lauren R Pepper; Ranganath Parthasarathy; Gregory P Robbins; Nicholas N Dang; Daniel A Hammer; Eric T Boder
Journal:  Protein Eng Des Sel       Date:  2013-06-19       Impact factor: 1.650

5.  Creation and Evaluation of a Single-chain Antibody Tetramer that Targets Brain Endothelial Cells.

Authors:  Xiaobin Zhang; Xin Xiang Wang; Eric V Shusta
Journal:  AIChE J       Date:  2014-04-01       Impact factor: 3.993

6.  Cellular-Based Selections Aid Yeast-Display Discovery of Genuine Cell-Binding Ligands: Targeting Oncology Vascular Biomarker CD276.

Authors:  Lawrence A Stern; Patrick S Lown; Alexandra C Kobe; Lotfi Abou-Elkacem; Juergen K Willmann; Benjamin J Hackel
Journal:  ACS Comb Sci       Date:  2019-01-24       Impact factor: 3.784

Review 7.  Glioblastoma cancer stem cells: Biomarker and therapeutic advances.

Authors:  Kelli B Pointer; Paul A Clark; Michael Zorniak; Bahauddeen M Alrfaei; John S Kuo
Journal:  Neurochem Int       Date:  2014-03-19       Impact factor: 3.921

8.  Keystone Symposium on Antibodies as Drugs: March 27-April 1, 2009, Whistler, BC CA.

Authors:  Thierry Wurch; Christel Larbouret; Bruno Robert
Journal:  MAbs       Date:  2009-07-28       Impact factor: 5.857

9.  Development of GFP-based biosensors possessing the binding properties of antibodies.

Authors:  Tej V Pavoor; Yong Ku Cho; Eric V Shusta
Journal:  Proc Natl Acad Sci U S A       Date:  2009-07-02       Impact factor: 11.205

10.  Magnetic Bead-Immobilized Mammalian Cells Are Effective Targets to Enrich Ligand-Displaying Yeast.

Authors:  Patrick S Lown; Benjamin J Hackel
Journal:  ACS Comb Sci       Date:  2020-04-27       Impact factor: 3.784
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.