L F Mataseje1, D A Boyd1, J Delport2, L Hoang3, M Imperial3, B Lefebvre4, M Kuhn5, P Van Caeseele6, B M Willey7, M R Mulvey8. 1. National Microbiology Laboratory, Winnipeg, MB, Canada. 2. London Health Sciences Centre, London, ON, Canada. 3. British Columbia Public Health Microbiology and Reference Laboratory, Vancouver, BC, Canada. 4. Laboratoire de santé publique du Québec, Sainte-Anne-de-Bellevue, QC, Canada. 5. Moncton Hospital, Moncton, NB, Canada. 6. Cadham Provincial Laboratory, Winnipeg, MB, Canada. 7. Mount Sinai Hospital, Toronto, ON, Canada. 8. National Microbiology Laboratory, Winnipeg, MB, Canada michael.mulvey@phac-aspc.gc.ca.
Abstract
OBJECTIVES: An increasing prevalence since 2010 of Serratia marcescens harbouring the Ambler class A carbapenemase SME prompted us to further characterize these isolates. METHODS: Isolates harbouring bla(SME) were identified by PCR and sequencing. Phenotypic analysis for carbapenemase activity was carried out by a modified Hodge test and a modified Carba NP test. Antimicrobial susceptibilities were determined by Etest and Vitek 2. Typing was by PFGE of macrorestriction digests. Whole-genome sequencing of three isolates was carried out to characterize the genomic region harbouring the bla(SME)-type genes. RESULTS: All S. marcescens harbouring SME-type enzymes could be detected using a modified Carba NP test. Isolates harbouring bla(SME) were resistant to penicillins and carbapenems, but remained susceptible to third-generation cephalosporins, as well as fluoroquinolones and trimethoprim/sulfamethoxazole. Isolates exhibited diverse genetic backgrounds, though 57% of isolates were found in three clusters. Analysis of whole-genome sequence data from three isolates revealed that the bla(SME) gene occurred in a novel cryptic prophage genomic island, SmarGI1-1. CONCLUSIONS: There has been an increasing occurrence of S. marcescens harbouring bla(SME) in Canada since 2010. The bla(SME) gene was found on a genomic island, SmarGI1-1, that can be excised and circularized, which probably contributes to its dissemination amongst S. marcescens.
OBJECTIVES: An increasing prevalence since 2010 of Serratia marcescens harbouring the Ambler class A carbapenemase SME prompted us to further characterize these isolates. METHODS: Isolates harbouring bla(SME) were identified by PCR and sequencing. Phenotypic analysis for carbapenemase activity was carried out by a modified Hodge test and a modified Carba NP test. Antimicrobial susceptibilities were determined by Etest and Vitek 2. Typing was by PFGE of macrorestriction digests. Whole-genome sequencing of three isolates was carried out to characterize the genomic region harbouring the bla(SME)-type genes. RESULTS: All S. marcescens harbouring SME-type enzymes could be detected using a modified Carba NP test. Isolates harbouring bla(SME) were resistant to penicillins and carbapenems, but remained susceptible to third-generation cephalosporins, as well as fluoroquinolones and trimethoprim/sulfamethoxazole. Isolates exhibited diverse genetic backgrounds, though 57% of isolates were found in three clusters. Analysis of whole-genome sequence data from three isolates revealed that the bla(SME) gene occurred in a novel cryptic prophage genomic island, SmarGI1-1. CONCLUSIONS: There has been an increasing occurrence of S. marcescens harbouring bla(SME) in Canada since 2010. The bla(SME) gene was found on a genomic island, SmarGI1-1, that can be excised and circularized, which probably contributes to its dissemination amongst S. marcescens.
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Authors: Laura F Mataseje; Kahina Abdesselam; Julie Vachon; Robyn Mitchel; Elizabeth Bryce; Diane Roscoe; David A Boyd; Joanne Embree; Kevin Katz; Pamela Kibsey; Andrew E Simor; Geoffrey Taylor; Nathalie Turgeon; Joanne Langley; Denise Gravel; Kanchana Amaratunga; Michael R Mulvey Journal: Antimicrob Agents Chemother Date: 2016-10-21 Impact factor: 5.191