Caroline M Mitchell1, Leslie McLemore2, Katharine Westerberg2, Rena Astronomo3, Kimberly Smythe3, Carolyn Gardella2, Matthias Mack4, Amalia Magaret5, Dorothy Patton2, Kathy Agnew2, M Juliana McElrath6, Florian Hladik7, David Eschenbach2. 1. Department of Obstetrics and Gynecology Fred Hutchinson Cancer Research Center, Vaccine and Infectious Diseases Division, Seattle, Washington. 2. Department of Obstetrics and Gynecology. 3. Fred Hutchinson Cancer Research Center, Vaccine and Infectious Diseases Division, Seattle, Washington. 4. Department of Internal Medicine II, University Hospital, Regensburg, Germany. 5. Department of Laboratory Medicine Fred Hutchinson Cancer Research Center, Vaccine and Infectious Diseases Division, Seattle, Washington. 6. Department of Laboratory Medicine Department of Medicine, Division of Allergy and Infectious Diseases Department of Global Health, University of Washington Fred Hutchinson Cancer Research Center, Vaccine and Infectious Diseases Division, Seattle, Washington. 7. Department of Obstetrics and Gynecology Department of Medicine, Division of Allergy and Infectious Diseases Fred Hutchinson Cancer Research Center, Vaccine and Infectious Diseases Division, Seattle, Washington.
Abstract
BACKGROUND: Depot medroxyprogesterone acetate (DMPA) has been linked to human immunodeficiency virus type 1 (HIV-1) acquisition. METHODS: Vaginal microbiota of women using DMPA for up to 2 years were cultured. Mucosal immune cell populations were measured by immunohistological staining. RESULTS: Over 12 months, the proportion with H2O2-positive lactobacilli decreased (n = 32; 53% vs 27%; P = .03). Median vaginal CD3(+) cells also decreased (n = 15; 355 vs 237 cells/mm(2); P = .03), as did CD3(+)CCR5(+) cells (195 vs 128 cells/mm(2); P = .04), HLA-DR(+) cells (130 vs 96 cells/mm(2); P = .27), and HLA-DR(+)CCR5(+) cells (18 vs 10 cells/mm(2); P = .33). CONCLUSIONS: DMPA contraception does not increase vaginal mucosal CCR5(+) HIV target cells but does decrease CD3(+) T lymphocytes and vaginal H2O2-producing lactobacilli.
BACKGROUND: Depot medroxyprogesterone acetate (DMPA) has been linked to human immunodeficiency virus type 1 (HIV-1) acquisition. METHODS: Vaginal microbiota of women using DMPA for up to 2 years were cultured. Mucosal immune cell populations were measured by immunohistological staining. RESULTS: Over 12 months, the proportion with H2O2-positive lactobacilli decreased (n = 32; 53% vs 27%; P = .03). Median vaginal CD3(+) cells also decreased (n = 15; 355 vs 237 cells/mm(2); P = .03), as did CD3(+)CCR5(+) cells (195 vs 128 cells/mm(2); P = .04), HLA-DR(+) cells (130 vs 96 cells/mm(2); P = .27), and HLA-DR(+)CCR5(+) cells (18 vs 10 cells/mm(2); P = .33). CONCLUSIONS:DMPA contraception does not increase vaginal mucosalCCR5(+) HIV target cells but does decrease CD3(+) T lymphocytes and vaginal H2O2-producing lactobacilli.
Authors: Vera Halpern; Stephanie L Combes; Laneta J Dorflinger; Debra H Weiner; David F Archer Journal: Contraception Date: 2013-07-12 Impact factor: 3.375
Authors: C K Mauck; M M Callahan; J Baker; K Arbogast; R Veazey; R Stock; Z Pan; C S Morrison; M Chen-Mok; D F Archer; H L Gabelnick Journal: Contraception Date: 1999-07 Impact factor: 3.375
Authors: P A Marx; A I Spira; A Gettie; P J Dailey; R S Veazey; A A Lackner; C J Mahoney; C J Miller; L E Claypool; D D Ho; N J Alexander Journal: Nat Med Date: 1996-10 Impact factor: 53.440
Authors: M J McElrath; K Smythe; J Randolph-Habecker; K R Melton; T A Goodpaster; S M Hughes; M Mack; A Sato; G Diaz; G Steinbach; R M Novak; Marcel E Curlin; M Curlin; J D Lord; J Maenza; A Duerr; N Frahm; Florian Hladik Journal: J Acquir Immune Defic Syndr Date: 2013-07-01 Impact factor: 3.731
Authors: E Saba; M Origoni; G Taccagni; D Ferrari; C Doglioni; A Nava; A Lisco; J-C Grivel; L Margolis; G Poli Journal: Mucosal Immunol Date: 2013-02-06 Impact factor: 7.313
Authors: Alison C Roxby; David N Fredricks; Katherine Odem-Davis; Kristjana Ásbjörnsdóttir; Linnet Masese; Tina L Fiedler; Stephen De Rosa; Walter Jaoko; James N Kiarie; Julie Overbaugh; R Scott McClelland Journal: J Acquir Immune Defic Syndr Date: 2016-04-01 Impact factor: 3.731
Authors: J Paul Brooks; David J Edwards; Diana L Blithe; Jennifer M Fettweis; Myrna G Serrano; Nihar U Sheth; Jerome F Strauss; Gregory A Buck; Kimberly K Jefferson Journal: Contraception Date: 2016-11-29 Impact factor: 3.375
Authors: Katherine G Michel; Richard P H Huijbregts; Jonathan L Gleason; Holly E Richter; Zdenek Hel Journal: J Acquir Immune Defic Syndr Date: 2015-04-15 Impact factor: 3.731