CONTEXT: Polycystic ovary syndrome (PCOS) is the most common cause of dysfunctional ovulation-affecting female fertility. A disintegrin and metalloproteinase with thrombospondin-like motifs (ADAMTS-1) is required for normal ovulation and subsequent fertilization, and the expression of ADAMTS-1 may be altered in PCOS granulosa cell (GC)-reflecting abnormalities in ovulatory signaling. OBJECTIVE: The purpose of this paper is to analyze the differential expression of ADAMTS-1 in PCOS patients associated with impaired oocyte quality. DESIGN AND SETTING: A prospective comparative experimental study was performed at a clinical reproductive medicine center. PATIENTS: Women with PCOS (n = 40) and normovulatory controls (n = 40) undergoing controlled ovarian hyperstimulation and in vitro fertilization were recruited in our study. MAIN OUTCOME MEASURES: Differential expression of ADAMTS-1 in GCs was analyzed with immunocytochemistry in PCOS patients and normal controls, and ADAMTS-1 mRNA expression was quantified by RT-PCR. Furthermore, the correlation between ADAMTS-1 mRNA and oocyte quality was analyzed. RESULTS: The expression of ADAMTS-1 was decreased in PCOS patients compared with normally ovulating women and was closely related to lower oocyte recovery, oocyte maturity, and fertilization rate. CONCLUSION: Our study provides evidence that the dysregulated expression of ADAMTS-1 in PCOS may influence oocyte quality-via GCs-oocyte paracrine and endocrine mechanism.
CONTEXT: Polycystic ovary syndrome (PCOS) is the most common cause of dysfunctional ovulation-affecting female fertility. A disintegrin and metalloproteinase with thrombospondin-like motifs (ADAMTS-1) is required for normal ovulation and subsequent fertilization, and the expression of ADAMTS-1 may be altered in PCOS granulosa cell (GC)-reflecting abnormalities in ovulatory signaling. OBJECTIVE: The purpose of this paper is to analyze the differential expression of ADAMTS-1 in PCOSpatients associated with impaired oocyte quality. DESIGN AND SETTING: A prospective comparative experimental study was performed at a clinical reproductive medicine center. PATIENTS: Women with PCOS (n = 40) and normovulatory controls (n = 40) undergoing controlled ovarian hyperstimulation and in vitro fertilization were recruited in our study. MAIN OUTCOME MEASURES: Differential expression of ADAMTS-1 in GCs was analyzed with immunocytochemistry in PCOSpatients and normal controls, and ADAMTS-1 mRNA expression was quantified by RT-PCR. Furthermore, the correlation between ADAMTS-1 mRNA and oocyte quality was analyzed. RESULTS: The expression of ADAMTS-1 was decreased in PCOSpatients compared with normally ovulating women and was closely related to lower oocyte recovery, oocyte maturity, and fertilization rate. CONCLUSION: Our study provides evidence that the dysregulated expression of ADAMTS-1 in PCOS may influence oocyte quality-via GCs-oocyte paracrine and endocrine mechanism.
Authors: M Karakose; K Demircan; E Tutal; T Demirci; M S Arslan; M Sahin; H T Celik; F Kazanci; J Karakaya; E Cakal; T Delibasi Journal: J Endocrinol Invest Date: 2016-05-04 Impact factor: 4.256
Authors: Minh Tam Le; Trung Van Nguyen; Tung Thanh Nguyen; Thai Thanh Thi Nguyen; Tam An Thi Nguyen; Quoc Huy Vu Nguyen; Ngoc Thanh Cao Journal: Eur J Obstet Gynecol Reprod Biol X Date: 2019-05-17