Marcos P Freire1, Daniel Pires2, Raphael Forjaz2, Sérgio Sato2, Ismael Cotrim3, Mônica Stiepcich4, Bruno Scarpellini4, José C Truzzi5. 1. Division of Urology, Fleury Group; Anhembi Morumbi Medical School and Federal University of Sao Paulo, SP, Brazil. 2. Anhembi Morumbi Medical School, Sao Paulo, SP, Brazil. 3. Division of Urology, Fleury Group and Federal University of Sao Paulo, Sao Paulo, SP, Brazil. 4. Division of Urology, Fleury Group, Sao Paulo, SP, Brazil. 5. Division of Urology, Fleury Group, Sao Paulo and Federal University of Sao Paulo, Sao Paulo, SP, Brazil.
Abstract
INTRODUCTION: HPV infection is a highly prevalent sexually transmitted disease and there is evidence of the relationship of HPV infection and the development of genital warts, penile intraepitelial neoplasia, invasive penile carcinoma and cervical cancer. However, there is sparse data regarding the prevalence of HPV types and co-infection of different HPV types among men. OBJECTIVES: To assess the prevalence of HPV subtypes infections and rates of co-infection among men. MATERIALS AND METHODS: 366 men were evaluated from March to October 2010. Men were referred to our institution for HPV diagnostic evaluation based on the following criteria: 1. presence of a genital wart; 2. presence of an atypical genital lesion; 3. absence of symptoms and a partner with a HPV diagnosis; 4. absence of symptoms and a desire to undergo a full STD diagnostic evaluation. Genital samples were collected from the urethra, penile shaft, scrotum and anus with Digene® collection and preservation kit and submitted to HPV genotype microarray detection (Papillocheck®). All men were tested for the low-risk HPV types 6-11-40-42-43-44 and for the high-risk HPV types 16-18-31-33-35-39-45-51-52-53-56-58-59-66-68-70-73-82. RESULTS: Of the 366 men, 11 were tested inconclusive and were excluded from the analysis. 256 men (72.1% of the men from the cohort referred to our institution) tested positive with genotype micro-array detection and 99 tested negative. The most preva¬lent HPV-subtypes in the studied population were 6, 42, 51 and 16. Co-infection was found in 153 men. Of those, 70 (19.7%) had a co-infection by 2 types, 37 (10.4%) by 3 types; 33 men (9.2%) by 4 types; 8 men (2.2%) by 5 types; 1 man (0.3%) by 6 types; 1 man (0.3%) by 7 types; 2 men (0.6%) by 8 types and 1 man (0.3%) by 9 types. CONCLUSION: The most frequent HPV types were 6, 16, 42 and 51. Co-infection was found in 59% of our patients. This information is vital to drive future public health policies including massive public vaccination campaign.
INTRODUCTION:HPV infection is a highly prevalent sexually transmitted disease and there is evidence of the relationship of HPV infection and the development of genital warts, penile intraepitelial neoplasia, invasive penile carcinoma and cervical cancer. However, there is sparse data regarding the prevalence of HPV types and co-infection of different HPV types among men. OBJECTIVES: To assess the prevalence of HPV subtypes infections and rates of co-infection among men. MATERIALS AND METHODS: 366 men were evaluated from March to October 2010. Men were referred to our institution for HPV diagnostic evaluation based on the following criteria: 1. presence of a genital wart; 2. presence of an atypical genital lesion; 3. absence of symptoms and a partner with a HPV diagnosis; 4. absence of symptoms and a desire to undergo a full STD diagnostic evaluation. Genital samples were collected from the urethra, penile shaft, scrotum and anus with Digene® collection and preservation kit and submitted to HPV genotype microarray detection (Papillocheck®). All men were tested for the low-risk HPV types 6-11-40-42-43-44 and for the high-risk HPV types 16-18-31-33-35-39-45-51-52-53-56-58-59-66-68-70-73-82. RESULTS: Of the 366 men, 11 were tested inconclusive and were excluded from the analysis. 256 men (72.1% of the men from the cohort referred to our institution) tested positive with genotype micro-array detection and 99 tested negative. The most preva¬lent HPV-subtypes in the studied population were 6, 42, 51 and 16. Co-infection was found in 153 men. Of those, 70 (19.7%) had a co-infection by 2 types, 37 (10.4%) by 3 types; 33 men (9.2%) by 4 types; 8 men (2.2%) by 5 types; 1 man (0.3%) by 6 types; 1 man (0.3%) by 7 types; 2 men (0.6%) by 8 types and 1 man (0.3%) by 9 types. CONCLUSION: The most frequent HPV types were 6, 16, 42 and 51. Co-infection was found in 59% of our patients. This information is vital to drive future public health policies including massive public vaccination campaign.
Authors: Xiao-Xian Xu; Jian-Song Zhou; Shu-Hui Yuan; Hua Yu; Han-Mei Lou Journal: Int J Environ Res Public Health Date: 2015-09-02 Impact factor: 3.390
Authors: Eric T Dawson; Sarah Wagner; David Roberson; Meredith Yeager; Joseph Boland; Erik Garrison; Stephen Chanock; Mark Schiffman; Tina Raine-Bennett; Thomas Lorey; Phillip E Castle; Lisa Mirabello; Richard Durbin Journal: BMC Bioinformatics Date: 2019-07-12 Impact factor: 3.169
Authors: Ari P Araujo-Neto; Hygor Ferreira-Fernandes; Carolina M M Amaral; Lina G Santos; Antônio C Freitas; Jacinto C Silva-Neto; Juan A Rey; Rommel R Burbano; Benedito B da Silva; France K N Yoshioka; Giovanny R Pinto Journal: Genet Mol Biol Date: 2016-03 Impact factor: 1.771