Literature DB >> 24637490

Engineering ribosomal leaky scanning and upstream open reading frames for precise control of protein translation.

Joshua P Ferreira1, William L Noderer1, Alexander J Diaz de Arce1, Clifford L Wang1.   

Abstract

We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also report on recent applications to our own research projects, including the coupling of uORF and translation initiation site (TIS) engineering with small molecule-inducible post-translational control. Finally, we discuss opportunities to investigate and potentially engineer gene-specific translational responses to cellular stress.

Keywords:  cellular stress; post-transcriptional regulation; ribosome scanning; translation initiation; uORF; upstream open reading frames

Mesh：

Substances：

Year:  2014        PMID： 24637490      PMCID： PMC4101011          DOI： 10.4161/bioe.27607

Source DB:  PubMed          Journal:  Bioengineered        ISSN： 2165-5979            Impact factor:   3.269


  34 in total

Review 1.  Regulation of translation initiation following stress.

Authors:  M S Sheikh; A J Fornace
Journal:  Oncogene       Date:  1999-11-01       Impact factor: 9.867

2.  Downstream secondary structure facilitates recognition of initiator codons by eukaryotic ribosomes.

Authors:  M Kozak
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

Review 3.  Structure-function insights into prokaryotic and eukaryotic translation initiation.

Authors:  Alexander G Myasnikov; Angelita Simonetti; Stefano Marzi; Bruno P Klaholz
Journal:  Curr Opin Struct Biol       Date:  2009-06-01       Impact factor: 6.809

4.  Tetracycline-regulated expression implemented through transcriptional activation combined with proximal and distal repression.

Authors:  Ryan W S Peacock; Kathryn A Sullivan; Clifford L Wang
Journal:  ACS Synth Biol       Date:  2012-04-24       Impact factor: 5.110

5.  Recognition of AUG and alternative initiator codons is augmented by G in position +4 but is not generally affected by the nucleotides in positions +5 and +6.

Authors:  M Kozak
Journal:  EMBO J       Date:  1997-05-01       Impact factor: 11.598

6.  Regulated translation initiation controls stress-induced gene expression in mammalian cells.

Authors:  H P Harding; I Novoa; Y Zhang; H Zeng; R Wek; M Schapira; D Ron
Journal:  Mol Cell       Date:  2000-11       Impact factor: 17.970

7.  Multiple upstream AUG codons mediate translational control of GCN4.

Authors:  P P Mueller; A G Hinnebusch
Journal:  Cell       Date:  1986-04-25       Impact factor: 41.582

8.  Effect of sequence context at stop codons on efficiency of reinitiation in GCN4 translational control.

Authors:  C M Grant; A G Hinnebusch
Journal:  Mol Cell Biol       Date:  1994-01       Impact factor: 4.272

9.  Requirements for intercistronic distance and level of eukaryotic initiation factor 2 activity in reinitiation on GCN4 mRNA vary with the downstream cistron.

Authors:  C M Grant; P F Miller; A G Hinnebusch
Journal:  Mol Cell Biol       Date:  1994-04       Impact factor: 4.272

10.  Optimization of oncogene expression through intra-population competition.

Authors:  Joshua P Ferreira; Clifford L Wang
Journal:  Biotechnol J       Date:  2013-08-05       Impact factor: 4.677
View more
  2 in total

1.  Basal p21 controls population heterogeneity in cycling and quiescent cell cycle states.

Authors:  K Wesley Overton; Sabrina L Spencer; William L Noderer; Tobias Meyer; Clifford L Wang
Journal:  Proc Natl Acad Sci U S A       Date:  2014-09-29       Impact factor: 11.205

2.  Genome-wide maps of ribosomal occupancy provide insights into adaptive evolution and regulatory roles of uORFs during Drosophila development.

Authors:  Hong Zhang; Shengqian Dou; Feng He; Junjie Luo; Liping Wei; Jian Lu
Journal:  PLoS Biol       Date:  2018-07-20       Impact factor: 8.029
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.