| Literature DB >> 24631480 |
Bartosz Wojtas1, Carolina Ferraz2, Tomasz Stokowy3, Steffen Hauptmann4, Dariusz Lange5, Henning Dralle6, Thomas Musholt7, Barbara Jarzab8, Ralf Paschke9, Markus Eszlinger10.
Abstract
The objective of the study was to identify microRNAs (miRs) characteristic for follicular thyroid carcinoma (FTC) and to define their role in tumorigenesis. A miR-microarray study was conducted to identify miRs differentially expressed between FTCs and their surrounding tissues. Selection was further reinforced by a literature review. Four miRs were selected and confirmed by RT-qPCR: miR-146b, -183, -221 were up-regulated, whereas miR-199b down-regulated in FTCs. The influence of these miRs on cell proliferation, cell cycle, apoptosis and migration was studied in HTori and FTC-133 cells. Functional characterization suggests an impact of miR-183 and miR-146b in FTC development. Overexpression of both miRs significantly induces migration. Moreover, overexpression of miR-183 significantly represses apoptosis. MiR-199b and -221 do not have significant effects on proliferation, cell cycle, apoptosis or migration in HTori and FTC-133 cells. Our data suggest that miR-146b and miR-183 may influence FTC development through the induction of migration and apoptosis inhibition.Entities:
Keywords: Apoptosis; Follicular thyroid cancer; Migration; miRNA
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Year: 2014 PMID： 24631480 DOI： 10.1016/j.mce.2014.02.011
Source DB: PubMed Journal: Mol Cell Endocrinol ISSN： 0303-7207 Impact factor: 4.102