Jie Cheng1, Fengsheng Li2, Jiuwei Cui1, Weiying Guo3, Cai Li1, Wei Li1, Guixia Wang1, Xiao Xing4, Ying Gao1, Yuanyuan Ge1, Guanjun Wang5, Lu Cai6. 1. The First Hospital of Jilin University, Changchun 130021, China. 2. Kosair Children's Hospital Research Institute, Department of Pediatrics, University of Louisville, Louisville, KY 40202, USA; The Second Artillery General Hospital, Beijing 1000884, China. 3. The First Hospital of Jilin University, Changchun 130021, China; Kosair Children's Hospital Research Institute, Department of Pediatrics, University of Louisville, Louisville, KY 40202, USA. 4. Changchun Institute for Food and Drug Control, Changchun 130000, China. 5. The First Hospital of Jilin University, Changchun 130021, China. Electronic address: guanjunw2006@163.com. 6. Kosair Children's Hospital Research Institute, Department of Pediatrics, University of Louisville, Louisville, KY 40202, USA; Department of Radiation Oncology and Pharmacology, University of Louisville, Louisville, KY 40202, USA; Department of Toxicology, University of Louisville, Louisville, KY 40202, USA. Electronic address: l0cai001@louisville.edu.
Abstract
AIMS: We reported the attenuation of diabetes-induced renal dysfunction by exposure to multiple low-dose radiation (LDR) at 25 mGy every other day by suppressing renal oxidative damage. We here explored the optimal conditions of LDR to protect the kidney from diabetes. MAIN METHODS: Male C57BL/6J mice with type 1 diabetes were induced with multiple injections of low-dose streptozotocin. Diabetic mice received whole body X-irradiation at a dose of 12.5, 25 or 50 mGy every other day for either 4 or 8 weeks. Age-matched normal mice were similarly irradiated at the dose of 25 mGy for 4 or 8 weeks. The renal function and histopathological changes were examined at the 4th and 8th weeks of the study. KEY FINDINGS: Diabetes induced renal dysfunction is shown by the decreased creatinine and increased microalbumin in the urine. Renal oxidative damage, detected by protein nitration and lipid oxidation, and remodeling, reflected by increased expression of connective tissue growth factor, collagen IV and fibronectin, were significantly increased in diabetic mice. All these renal pathological and function changes in diabetic mice were significantly attenuated by exposure to LDR at all regimens, among which, however, exposure to LDR at 12.5 mGy for 8 weeks provided the best protective effect on the kidney of diabetic mice. SIGNIFICANCE: Our results suggest that whole-body LDR at 12.5 mGy every other day for 8 weeks is the optimal condition of LDR to protect the kidney from diabetes.
AIMS: We reported the attenuation of diabetes-induced renal dysfunction by exposure to multiple low-dose radiation (LDR) at 25 mGy every other day by suppressing renal oxidative damage. We here explored the optimal conditions of LDR to protect the kidney from diabetes. MAIN METHODS: Male C57BL/6J mice with type 1 diabetes were induced with multiple injections of low-dose streptozotocin. Diabeticmice received whole body X-irradiation at a dose of 12.5, 25 or 50 mGy every other day for either 4 or 8 weeks. Age-matched normal mice were similarly irradiated at the dose of 25 mGy for 4 or 8 weeks. The renal function and histopathological changes were examined at the 4th and 8th weeks of the study. KEY FINDINGS:Diabetes induced renal dysfunction is shown by the decreased creatinine and increased microalbumin in the urine. Renal oxidative damage, detected by protein nitration and lipid oxidation, and remodeling, reflected by increased expression of connective tissue growth factor, collagen IV and fibronectin, were significantly increased in diabeticmice. All these renal pathological and function changes in diabeticmice were significantly attenuated by exposure to LDR at all regimens, among which, however, exposure to LDR at 12.5 mGy for 8 weeks provided the best protective effect on the kidney of diabeticmice. SIGNIFICANCE: Our results suggest that whole-body LDR at 12.5 mGy every other day for 8 weeks is the optimal condition of LDR to protect the kidney from diabetes.
Authors: Chi Zhang; Yi Tan; Weiying Guo; Cai Li; Shunzi Ji; Xiaokun Li; Lu Cai Journal: Am J Physiol Endocrinol Metab Date: 2009-09-29 Impact factor: 4.310