Manuel Toledano1, Fátima S Aguilera2, Salvatore Sauro3, Inmaculada Cabello2, Estrella Osorio2, Raquel Osorio2. 1. University of Granada, Faculty of Dentistry, Dental Materials Section, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain. Electronic address: toledano@ugr.es. 2. University of Granada, Faculty of Dentistry, Dental Materials Section, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain. 3. Biomaterials, Biomimetics and Biophotonics King's College London Dental Institute, Guy' Dental Hospital, Central Office, Floor 18, Guy's Tower, London SE1 9RT, UK.
Abstract
OBJECTIVES: The purpose of this study was to evaluate if mechanical loading promotes bioactivity at the resin interface after bonding with three different adhesive approaches. METHODS: Dentin surfaces were subjected to three different treatments: demineralisation by (1) 37% phosphoric acid (PA) followed by application of an etch-and-rinse dentin adhesive Single Bond (SB) (PA+SB), (2) by 0.5 M ethylenediaminetetraacetic acid (EDTA) followed by SB (EDTA+SB), (3) application of a self-etch dentin adhesive: Clearfil SE Bond (SEB). Bonded interfaces were stored in simulated body fluid during 24 h or 3w. One half of each tooth was submitted to mechanical loading. Remineralisation of the bonded interfaces was assessed by AFM imaging/nano-indentation, Raman spectroscopy/cluster analysis, dye assisted confocal microscopy evaluation (CLSM) and Masson's trichrome staining. RESULTS: Loading cycling for 3w promoted an increase of mechanical properties at the resin-dentin interface. Cluster analysis demonstrated an augmentation of the mineral-matrix ratio in SB-loaded specimens. CLSM showed an absent micropermeability and nanoleakage after loading EDTA+SB and SEB specimens. Trichrome staining reflected a narrow demineralised dentin matrix after loading, almost not observable in EDTA+SB and SEB. SIGNIFICANCE: In vitro mechanical loading promoted mineralization in the resin-dentin interfaces, at 24 h and 3w of storage.
OBJECTIVES: The purpose of this study was to evaluate if mechanical loading promotes bioactivity at the resin interface after bonding with three different adhesive approaches. METHODS: Dentin surfaces were subjected to three different treatments: demineralisation by (1) 37% phosphoric acid (PA) followed by application of an etch-and-rinse dentin adhesive Single Bond (SB) (PA+SB), (2) by 0.5 M ethylenediaminetetraacetic acid (EDTA) followed by SB (EDTA+SB), (3) application of a self-etch dentin adhesive: Clearfil SE Bond (SEB). Bonded interfaces were stored in simulated body fluid during 24 h or 3w. One half of each tooth was submitted to mechanical loading. Remineralisation of the bonded interfaces was assessed by AFM imaging/nano-indentation, Raman spectroscopy/cluster analysis, dye assisted confocal microscopy evaluation (CLSM) and Masson's trichrome staining. RESULTS: Loading cycling for 3w promoted an increase of mechanical properties at the resin-dentin interface. Cluster analysis demonstrated an augmentation of the mineral-matrix ratio in SB-loaded specimens. CLSM showed an absent micropermeability and nanoleakage after loading EDTA+SB and SEB specimens. Trichrome staining reflected a narrow demineralised dentin matrix after loading, almost not observable in EDTA+SB and SEB. SIGNIFICANCE: In vitro mechanical loading promoted mineralization in the resin-dentin interfaces, at 24 h and 3w of storage.
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