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Literature DB >> 24630106

Generation of differentially modified microtubules using in vitro enzymatic approaches.

Annapurna Vemu¹, Christopher P Garnham¹, Duck-Yeon Lee², Antonina Roll-Mecak³.

Abstract

Tubulin, the building block of microtubules, is subject to chemically diverse and evolutionarily conserved post-translational modifications that mark microtubules for specific functions in the cell. Here we describe in vitro methods for generating homogenous acetylated, glutamylated, or tyrosinated tubulin and microtubules using recombinantly expressed and purified modification enzymes. The generation of differentially modified microtubules now enables a mechanistic dissection of the effects of tubulin post-translational modifications on the dynamics and mechanical properties of microtubules as well as the behavior of motors and microtubule-associated proteins.

Entities: CellLine Chemical Disease Gene Species

Keywords: Microtubule; Motors; Tubulin acetylation; Tubulin acetyltransferase (α-TAT); Tubulin glutamylation; Tubulin post-translational modifications; Tubulin tyrosination; Tubulin tyrosine ligase (TTL); Tubulin tyrosine ligase-like (TTLL)

Mesh：

Substances：

Year: 2014 PMID： 24630106 PMCID： PMC7416453 DOI： 10.1016/B978-0-12-397924-7.00009-1

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

42 in total

1. Class II tubulin, the major brain beta tubulin isotype is polyglutamylated on glutamic acid residue 435.

Authors: M Rüdiger; U Plessman; K D Klöppel; J Wehland; K Weber
Journal: FEBS Lett Date: 1992-08-10 Impact factor: 4.124

2. Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function.

Authors: Koji Ikegami; Robb L Heier; Midori Taruishi; Hiroshi Takagi; Masahiro Mukai; Shuichi Shimma; Shu Taira; Ken Hatanaka; Nobuhiro Morone; Ikuko Yao; Patrick K Campbell; Shigeki Yuasa; Carsten Janke; Grant R Macgregor; Mitsutoshi Setou
Journal: Proc Natl Acad Sci U S A Date: 2007-02-20 Impact factor: 11.205

3. Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency.

Authors: R B Kapust; J Tözsér; J D Fox; D E Anderson; S Cherry; T D Copeland; D S Waugh
Journal: Protein Eng Date: 2001-12

4. High-resolution model of the microtubule.

Authors: E Nogales; M Whittaker; R A Milligan; K H Downing
Journal: Cell Date: 1999-01-08 Impact factor: 41.582

Review 5. Structure and utilization of tubulin isotypes.

Authors: K F Sullivan
Journal: Annu Rev Cell Biol Date: 1988

6. A soluble preparation from rat brain that incorporates into its own proteins ( 14 C)arginine by a ribonuclease-sensitive system and ( 14 C)tyrosine by a ribonuclease-insensitive system.

Authors: H S Barra; J A Rodriguez; C A Arce; R Caputto
Journal: J Neurochem Date: 1973-01 Impact factor: 5.372

Review 7. Methods in tubulin proteomics.

Authors: Leah M Miller; Hui Xiao; Berta Burd; Susan Band Horwitz; Ruth Hogue Angeletti; Pascal Verdier-Pinard
Journal: Methods Cell Biol Date: 2010 Impact factor: 1.441

8. Posttranslational acetylation of α-tubulin constrains protofilament number in native microtubules.

Authors: Juan G Cueva; Jen Hsin; Kerwyn Casey Huang; Miriam B Goodman
Journal: Curr Biol Date: 2012-05-31 Impact factor: 10.834

9. The major alpha-tubulin K40 acetyltransferase alphaTAT1 promotes rapid ciliogenesis and efficient mechanosensation.

Authors: Toshinobu Shida; Juan G Cueva; Zhenjie Xu; Miriam B Goodman; Maxence V Nachury
Journal: Proc Natl Acad Sci U S A Date: 2010-11-10 Impact factor: 11.205

10. Tubulin tyrosine ligase structure reveals adaptation of an ancient fold to bind and modify tubulin.

Authors: Agnieszka Szyk; Alexandra M Deaconescu; Grzegorz Piszczek; Antonina Roll-Mecak
Journal: Nat Struct Mol Biol Date: 2011-10-23 Impact factor: 15.369

17 in total

Review 1. Writing and Reading the Tubulin Code.

Authors: Ian Yu; Christopher P Garnham; Antonina Roll-Mecak
Journal: J Biol Chem Date: 2015-05-08 Impact factor: 5.157

2. Katanin Grips the β-Tubulin Tail through an Electropositive Double Spiral to Sever Microtubules.

Authors: Elena A Zehr; Agnieszka Szyk; Ewa Szczesna; Antonina Roll-Mecak
Journal: Dev Cell Date: 2019-11-14 Impact factor: 12.270

3. Crystal structure of tubulin tyrosine ligase-like 3 reveals essential architectural elements unique to tubulin monoglycylases.

Authors: Christopher P Garnham; Ian Yu; Yan Li; Antonina Roll-Mecak
Journal: Proc Natl Acad Sci U S A Date: 2017-06-02 Impact factor: 11.205

Generation of differentially modified microtubules using in vitro enzymatic approaches.

1. Class II tubulin, the major brain beta tubulin isotype is polyglutamylated on glutamic acid residue 435.

2. Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function.

3. Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency.

4. High-resolution model of the microtubule.

Review 5. Structure and utilization of tubulin isotypes.

6. A soluble preparation from rat brain that incorporates into its own proteins ( 14 C)arginine by a ribonuclease-sensitive system and ( 14 C)tyrosine by a ribonuclease-insensitive system.

Review 7. Methods in tubulin proteomics.

8. Posttranslational acetylation of α-tubulin constrains protofilament number in native microtubules.

9. The major alpha-tubulin K40 acetyltransferase alphaTAT1 promotes rapid ciliogenesis and efficient mechanosensation.

10. Tubulin tyrosine ligase structure reveals adaptation of an ancient fold to bind and modify tubulin.

Review 1. Writing and Reading the Tubulin Code.

2. Katanin Grips the β-Tubulin Tail through an Electropositive Double Spiral to Sever Microtubules.

3. Crystal structure of tubulin tyrosine ligase-like 3 reveals essential architectural elements unique to tubulin monoglycylases.

4. Multivalent Microtubule Recognition by Tubulin Tyrosine Ligase-like Family Glutamylases.

5. Molecular Determinants of Tubulin's C-Terminal Tail Conformational Ensemble.

Review 6. Intrinsically disordered tubulin tails: complex tuners of microtubule functions?

Review 7. Posttranslational modifications of the cytoskeleton.

8. C-Terminal Tail Polyglycylation and Polyglutamylation Alter Microtubule Mechanical Properties.

9. Structural basis for polyglutamate chain initiation and elongation by TTLL family enzymes.

10. Genetically encoded live-cell sensor for tyrosinated microtubules.