| Literature DB >> 24625190 |
Shu-Shan Gao1, Joanne Hothersall, Ji'en Wu, Annabel C Murphy, Zhongshu Song, Elton R Stephens, Christopher M Thomas, Matthew P Crump, Russell J Cox, Thomas J Simpson, Christine L Willis.
Abstract
Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.Entities:
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Year: 2014 PMID： 24625190 DOI： 10.1021/ja501731p
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419