| Literature DB >> 24622388 |
Qiuhao Qu1, Dong Li1, Kathleen R Louis2, Xiangzhen Li3, Hong Yang1, Qinyu Sun1, Shane R Crandall2, Stephanie Tsang1, Jiaxi Zhou4, Charles L Cox2, Jianjun Cheng5, Fei Wang1.
Abstract
Efficient derivation of large-scale motor neurons (MNs) from human pluripotent stem cells is central to the understanding of MN development, modelling of MN disorders in vitro and development of cell-replacement therapies. Here we develop a method for rapid (20 days) and highly efficient (~70%) differentiation of mature and functional MNs from human pluripotent stem cells by tightly modulating neural patterning temporally at a previously undefined primitive neural progenitor stage. This method also allows high-yield (>250%) MN production in chemically defined adherent cultures. Furthermore, we show that Islet-1 is essential for formation of mature and functional human MNs, but, unlike its mouse counterpart, does not regulate cell survival or suppress the V2a interneuron fate. Together, our discoveries improve the strategy for MN derivation, advance our understanding of human neural specification and MN development, and provide invaluable tools for human developmental studies, drug discovery and regenerative medicine.Entities:
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Year: 2014 PMID： 24622388 DOI： 10.1038/ncomms4449
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919