Literature DB >> 24614370

Quantification of cell-associated atazanavir, darunavir, lopinavir, ritonavir, and efavirenz concentrations in human mononuclear cell extracts.

Anthony T Podany1, Lee C Winchester, Brian L Robbins, Courtney V Fletcher.   

Abstract

An ultrasensitive assay utilizing high-pressure liquid chromatography and mass spectrometry detection was developed and validated for the quantification of the antiretrovirals atazanavir (ATV), darunavir (DRV), lopinavir (LPV), ritonavir (RTV), and efavirenz (EFV) in human mononuclear cell (MNC) extracts. The assay utilizes 20 μl of cellular extract that contains as few as 50,000 MNCs. The analytical range of the assay is 0.0200 to 10.0 fmol/μl for ATV, 0.0500 to 25.0 fmol/μl for DRV, LPV, and RTV, and 0.200 to 100 fmol/μl for EFV. The assay has proven to be a clinically useful tool for investigating antiretroviral drug concentrations in virologic sanctuaries where harvested cell numbers are extremely low. The assay provides a tool for investigators to explore the clinical pharmacology of strategies for prevention, treatment, and cure in pathophysiologically relevant sites.

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Year:  2014        PMID： 24614370      PMCID： PMC3993270          DOI： 10.1128/AAC.02551-13

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  16 in total

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Authors:  B K Matuszewski; M L Constanzer; C M Chavez-Eng
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Review 2.  Tissue concentrations: do we ever learn?

Authors:  Johan W Mouton; Ursula Theuretzbacher; William A Craig; Paul M Tulkens; Hartmut Derendorf; Otto Cars
Journal:  J Antimicrob Chemother       Date:  2007-12-06       Impact factor: 5.790

3.  Persistent HIV-1 replication is associated with lower antiretroviral drug concentrations in lymphatic tissues.

Authors:  Courtney V Fletcher; Kathryn Staskus; Stephen W Wietgrefe; Meghan Rothenberger; Cavan Reilly; Jeffrey G Chipman; Greg J Beilman; Alexander Khoruts; Ann Thorkelson; Thomas E Schmidt; Jodi Anderson; Katherine Perkey; Mario Stevenson; Alan S Perelson; Daniel C Douek; Ashley T Haase; Timothy W Schacker
Journal:  Proc Natl Acad Sci U S A       Date:  2014-01-27       Impact factor: 11.205

4.  The gastrointestinal tract is critical to the pathogenesis of acute HIV-1 infection.

Authors:  Saurabh Mehandru; Klara Tenner-Racz; Paul Racz; Martin Markowitz
Journal:  J Allergy Clin Immunol       Date:  2005-08       Impact factor: 10.793

5.  Kinetics of antiviral activity and intracellular pharmacokinetics of human immunodeficiency virus type 1 protease inhibitors in tissue culture.

Authors:  M Nascimbeni; C Lamotte; G Peytavin; R Farinotti; F Clavel
Journal:  Antimicrob Agents Chemother       Date:  1999-11       Impact factor: 5.191

6.  Lymphoid organs function as major reservoirs for human immunodeficiency virus.

Authors:  G Pantaleo; C Graziosi; L Butini; P A Pizzo; S M Schnittman; D P Kotler; A S Fauci
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-01       Impact factor: 11.205

7.  Steady-state pharmacokinetics of abacavir in plasma and intracellular carbovir triphosphate following administration of abacavir at 600 milligrams once daily and 300 milligrams twice daily in human immunodeficiency virus-infected subjects.

Authors:  Graeme Moyle; Marta Boffito; Carl Fletcher; Chris Higgs; Phillip E Hay; Ivy H Song; Yu Lou; Geoffrey J Yuen; Sherene S Min; Elena M Guerini
Journal:  Antimicrob Agents Chemother       Date:  2009-02-02       Impact factor: 5.191

8.  Intracellular and plasma pharmacokinetics of saquinavir-ritonavir, administered at 1,600/100 milligrams once daily in human immunodeficiency virus-infected patients.

Authors:  Jennifer Ford; Marta Boffito; Adrian Wildfire; Andrew Hill; David Back; Saye Khoo; Mark Nelson; Graeme Moyle; Brian Gazzard; Anton Pozniak
Journal:  Antimicrob Agents Chemother       Date:  2004-07       Impact factor: 5.191

9.  Persistence of HIV in gut-associated lymphoid tissue despite long-term antiretroviral therapy.

Authors:  Tae-Wook Chun; David C Nickle; Jesse S Justement; Jennifer H Meyers; Gregg Roby; Claire W Hallahan; Shyam Kottilil; Susan Moir; Joann M Mican; James I Mullins; Douglas J Ward; Joseph A Kovacs; Peter J Mannon; Anthony S Fauci
Journal:  J Infect Dis       Date:  2008-03-01       Impact factor: 5.226

10.  Primary HIV-1 infection is associated with preferential depletion of CD4+ T lymphocytes from effector sites in the gastrointestinal tract.

Authors:  Saurabh Mehandru; Michael A Poles; Klara Tenner-Racz; Amir Horowitz; Arlene Hurley; Christine Hogan; Daniel Boden; Paul Racz; Martin Markowitz
Journal:  J Exp Med       Date:  2004-09-13       Impact factor: 14.307
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  9 in total

1.  Alterations in P-Glycoprotein Expression and Function Between Macrophage Subsets.

Authors:  Theodore J Cory; Hui He; Lee C Winchester; Santosh Kumar; Courtney V Fletcher
Journal:  Pharm Res       Date:  2016-07-18       Impact factor: 4.200

2.  Mass spectrometry imaging reveals heterogeneous efavirenz distribution within putative HIV reservoirs.

Authors:  Corbin G Thompson; Mark T Bokhart; Craig Sykes; Lourdes Adamson; Yuri Fedoriw; Paul A Luciw; David C Muddiman; Angela D M Kashuba; Elias P Rosen
Journal:  Antimicrob Agents Chemother       Date:  2015-03-02       Impact factor: 5.191

3.  A rapid spin through oil results in higher cell-associated concentrations of antiretrovirals compared with conventional cell washing.

Authors:  Theodore J Cory; Lee C Winchester; Brian L Robbins; Courtney V Fletcher
Journal:  Bioanalysis       Date:  2015       Impact factor: 2.681

4.  Assessing the lymphoid tissue bioavailability of antiretrovirals in human primary lymphoid endothelial cells and in mice.

Authors:  Shetty Ravi Dyavar; Nagsen Gautam; Anthony T Podany; Lee C Winchester; Jonathan A Weinhold; Timothy M Mykris; Kayla M Campbell; Yazen Alnouti; Courtney V Fletcher
Journal:  J Antimicrob Chemother       Date:  2019-10-01       Impact factor: 5.790

5.  Persistent HIV transcription and variable antiretroviral drug penetration in lymph nodes during plasma viral suppression.

Authors:  Courtney V Fletcher; Eugène Kroon; Timothy Schacker; Suteeraporn Pinyakorn; Nicolas Chomont; Suthat Chottanapund; Peeriya Prueksakaew; Khunthalee Benjapornpong; Supranee Buranapraditkun; Nittaya Phanuphak; Jintanat Ananworanich; Sandhya Vasan; Denise Hsu
Journal:  AIDS       Date:  2022-02-18       Impact factor: 4.632

6.  Defining total-body AIDS-virus burden with implications for curative strategies.

Authors:  Jacob D Estes; Cissy Kityo; Francis Ssali; Louise Swainson; Krystelle Nganou Makamdop; Gregory Q Del Prete; Steven G Deeks; Paul A Luciw; Jeffrey G Chipman; Gregory J Beilman; Torfi Hoskuldsson; Alexander Khoruts; Jodi Anderson; Claire Deleage; Jacob Jasurda; Thomas E Schmidt; Michael Hafertepe; Samuel P Callisto; Hope Pearson; Thomas Reimann; Jared Schuster; Jordan Schoephoerster; Peter Southern; Katherine Perkey; Liang Shang; Stephen W Wietgrefe; Courtney V Fletcher; Jeffrey D Lifson; Daniel C Douek; Joseph M McCune; Ashley T Haase; Timothy W Schacker
Journal:  Nat Med       Date:  2017-10-02       Impact factor: 53.440

7.  Impact of Integrase Inhibition Compared With Nonnucleoside Inhibition on HIV Reservoirs in Lymphoid Tissues.

Authors:  Meghan Rothenberger; Krystelle Nganou-Makamdop; Cissy Kityo; Francis Ssali; Jeffrey G Chipman; Gregory J Beilman; Torfi Hoskuldsson; Jodi Anderson; Jake Jasurda; Thomas E Schmidt; Samuel P Calisto; Hope Pearson; Thomas Reimann; Caitlin David; Katherine Perkey; Peter Southern; Steve Wietgrefe; Erika Helgeson; Cavan Reilly; Ashley T Haase; Daniel C Douek; Courtney V Fletcher; Timothy W Schacker
Journal:  J Acquir Immune Defic Syndr       Date:  2019-07-01       Impact factor: 3.731

8.  A Versatile Method to Determine the Cellular Bioavailability of Small-Molecule Inhibitors.

Authors:  Kevin B Teuscher; Min Zhang; Haitao Ji
Journal:  J Med Chem       Date:  2017-01-03       Impact factor: 7.446

9.  Transplantation of CCR5∆32 Homozygous Umbilical Cord Blood in a Child With Acute Lymphoblastic Leukemia and Perinatally Acquired HIV Infection.

Authors:  Meghan Rothenberger; John E Wagner; Ashley Haase; Douglas Richman; Bartosz Grzywacz; Matthew Strain; Steven Lada; Jacob Estes; Courtney V Fletcher; Anthony T Podany; Jodi Anderson; Thomas Schmidt; Steve Wietgrefe; Timothy Schacker; Michael R Verneris
Journal:  Open Forum Infect Dis       Date:  2018-05-22       Impact factor: 3.835
  9 in total

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