| Literature DB >> 24613616 |
Lu Zhang1, Aya Nomura-Kitabayashi1, Nishat Sultana1, Weibin Cai1, Xiaoqiang Cai1, Anne M Moon2, Chen-Leng Cai3.
Abstract
The vertebrate heart develops from mesoderm and requires inductive signals secreted from early endoderm. During embryogenesis, Nkx2.5 acts as a key transcription factor and plays essential roles for heart formation from Drosophila to human. In mice, Nkx2.5 is expressed in the early first heart field, second heart field pharyngeal mesoderm, as well as pharyngeal endodermal cells underlying the second heart field. Currently, the specific requirements for Nkx2.5 in the endoderm versus mesoderm with regard to early heart formation are incompletely understood. Here, we performed tissue-specific deletion in mice to dissect the roles of Nkx2.5 in the pharyngeal endoderm and mesoderm. We found that heart development appeared normal after endodermal deletion of Nkx2.5 whereas mesodermal deletion engendered cardiac defects almost identical to those observed on Nkx2.5 null embryos (Nkx2.5(-/-)). Furthermore, re-expression of Nkx2.5 in the mesoderm rescued Nkx2.5(-/-) heart defects. Our findings reveal that Nkx2.5 in the mesoderm is essential while endodermal expression is dispensable for early heart formation in mammals.Entities:
Keywords: Heart development; Nkx2.5; Pharyngeal endoderm; Pharyngeal mesoderm; Second heart field
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Year: 2014 PMID： 24613616 PMCID： PMC4461860 DOI： 10.1016/j.ydbio.2014.02.023
Source DB: PubMed Journal: Dev Biol ISSN： 0012-1606 Impact factor: 3.582