Literature DB >> 2461113

The use of polyvinylidenedifluoride membranes as a general blotting matrix.

H Gültekin1, K H Heermann.   

Abstract

A fast chemical staining of protein by Coomassie blue on a stable nonshrinking membrane of polyvinylidenedifluoride is described. The procedure is especially useful for detection of nonlabeled marker proteins in comparison to specifically detected proteins. The membrane is highly suitable for other chemical stains like silver, gold, or india ink for immunostaining, lectin binding, and binding of radioactive ligands like 45Ca. Blocking of the background against nonspecific binding to proteins was studied using different reagents. A multiple slit plate is described for volume-saving simultaneous application of multiple reactants on one protein-loaded membrane.

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Year:  1988        PMID： 2461113     DOI： 10.1016/0003-2697(88)90451-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  18 in total

1.  Identification of an immunoreactive non-proteinaleous component in Borrelia burgdorferi.

Authors:  H Eiffert; H Lotter; K Jarecki-Khan; R Thomssen
Journal:  Med Microbiol Immunol       Date:  1991       Impact factor: 3.402

2.  Epidermal growth factor receptor, but not c-erbB-2, activation prevents lactogenic hormone induction of the beta-casein gene in mouse mammary epithelial cells.

Authors:  N E Hynes; D Taverna; I M Harwerth; F Ciardiello; D S Salomon; T Yamamoto; B Groner
Journal:  Mol Cell Biol       Date:  1990-08       Impact factor: 4.272

3.  Pherophorins: a family of extracellular matrix glycoproteins from Volvox structurally related to the sex-inducing pheromone.

Authors:  K Godl; A Hallmann; A Rappel; M Sumper
Journal:  Planta       Date:  1995       Impact factor: 4.116

4.  Cationic glycoproteins in sera of patients with acute infections identified as kappa light chain glycosylated IgG.

Authors:  K Ritter; A Wiebusch; M Mäder; H Eiffert; H Kratzin; R Thomssen
Journal:  Med Microbiol Immunol       Date:  1993-07       Impact factor: 3.402

5.  A cytosol derived factor of Group B streptococcus prevent its invasion into human epithelial cells.

Authors:  Manju Ohri; Smriti Parashar; Venkatesh S Pai; Sujata Ghosh; Anuradha Chakraborti
Journal:  World J Microbiol Biotechnol       Date:  2018-03-08       Impact factor: 3.312

6.  Nucleotide sequence of the ospAB operon of a Borrelia burgdorferi strain expressing OspA but not OspB.

Authors:  H Eiffert; A Ohlenbusch; W Fehling; H Lotter; R Thomssen
Journal:  Infect Immun       Date:  1992-05       Impact factor: 3.441

7.  Insulin-like growth factor I receptors of fetal brain are enriched in nerve growth cones and contain a beta-subunit variant.

Authors:  S Quiroga; R S Garofalo; K H Pfenninger
Journal:  Proc Natl Acad Sci U S A       Date:  1995-05-09       Impact factor: 11.205

8.  The 39-kilodalton protein of Borrelia burgdorferi: a target for bactericidal human monoclonal antibodies.

Authors:  M Scriba; J S Ebrahim; T Schlott; H Eiffert
Journal:  Infect Immun       Date:  1993-10       Impact factor: 3.441

9.  Analysis of the tumour suppressor genes, FHIT and WT-1, and the tumour rejection genes, BAGE, GAGE-1/2, HAGE, MAGE-1, and MAGE-3, in benign and malignant neoplasms of the salivary glands.

Authors:  H Nagel; R Laskawi; H Eiffert; T Schlott
Journal:  Mol Pathol       Date:  2003-08

10.  Protein C inhibitor in human body fluids. Seminal plasma is rich in inhibitor antigen deriving from cells throughout the male reproductive system.

Authors:  M Laurell; A Christensson; P A Abrahamsson; J Stenflo; H Lilja
Journal:  J Clin Invest       Date:  1992-04       Impact factor: 14.808
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