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Literature DB >> 2460876

M2 delta, a candidate for the structure lining the ionic channel of the nicotinic cholinergic receptor.

Abstract

A synthetic 23-mer peptide that mimics the sequence of the putative transmembrane M2 segment of the Torpedo californica acetylcholine receptor (AcChoR) delta subunit--Glu-Lys-Met-Ser-Thr-Ala-Ile-Ser-Val-Leu-Leu-Ala-Gln-Ala-Val-Phe-Leu- Leu-Leu-Thr-Ser-Gln-Arg--forms discrete ionic channels in phosphatidylcholine bilayers. In contrast, a synthetic peptide that mimics the sequence of the putative M1 transmembrane segment of the Torpedo AcChoR delta subunit--Leu-Phe-Tyr-Val-Ile-Asn-Phe-Ile-Thr-Pro-Cys-Val-Leu-Ile-Ser-Phe- Leu-Ala-Ser-Leu-Ala-Phe-Tyr--does not form channels. The synthetic M2 delta channel peptide exhibits features that are characteristic of the authentic AcChoR channel, such as single channel conductances, discrimination of cations over anions, and channel lifetimes for open and closed states in the millisecond time range. Energetic considerations suggest that an aggregate of five amphipathic alpha-helices conforms the channel. Thus, the M2 segment may be a component of the AcChoR channel structure.

Entities: Chemical Species

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Year: 1988 PMID： 2460876 PMCID： PMC282529 DOI： 10.1073/pnas.85.22.8703

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

33 in total

Review 1. Prediction of the secondary structure of proteins from their amino acid sequence.

Authors: P Y Chou; G D Fasman
Journal: Adv Enzymol Relat Areas Mol Biol Date: 1978

2. Primary structure of alpha-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequence.

Authors: M Noda; H Takahashi; T Tanabe; M Toyosato; Y Furutani; T Hirose; M Asai; S Inayama; T Miyata; S Numa
Journal: Nature Date: 1982-10-28 Impact factor: 49.962

3. Structural and functional diversity in 4-alpha-helical proteins.

Authors: P C Weber; F R Salemme
Journal: Nature Date: 1980-09-04 Impact factor: 49.962

4. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.

Authors: J Garnier; D J Osguthorpe; B Robson
Journal: J Mol Biol Date: 1978-03-25 Impact factor: 5.469

5. Helix to helix packing in proteins.

Authors: C Chothia; M Levitt; D Richardson
Journal: J Mol Biol Date: 1981-01-05 Impact factor: 5.469

6. Molecular weight in detergent solution of acetylcholine receptor from Torpedo californica.

Authors: J A Reynolds; A Karlin
Journal: Biochemistry Date: 1978-05-30 Impact factor: 3.162

7. Primary structures of beta- and delta-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequences.

Authors: M Noda; H Takahashi; T Tanabe; M Toyosato; S Kikyotani; T Hirose; M Asai; H Takashima; S Inayama; T Miyata; S Numa
Journal: Nature Date: 1983-01-20 Impact factor: 49.962

M2 delta, a candidate for the structure lining the ionic channel of the nicotinic cholinergic receptor.

Review 1. Prediction of the secondary structure of proteins from their amino acid sequence.

2. Primary structure of alpha-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequence.

3. Structural and functional diversity in 4-alpha-helical proteins.

4. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.

5. Helix to helix packing in proteins.

6. Molecular weight in detergent solution of acetylcholine receptor from Torpedo californica.

7. Primary structures of beta- and delta-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequences.

8. The permeability of endplate channels to monovalent and divalent metal cations.

9. Selectivity of cations and nonelectrolytes for acetylcholine-activated channels in cultured muscle cells.

10. The permeability of the endplate channel to organic cations in frog muscle.

1. Molecular dynamics of synthetic leucine-serine ion channels in a phospholipid membrane.

2. A model of the closed form of the nicotinic acetylcholine receptor m2 channel pore.

3. Purification and functional reconstitution of N- and C-halves of the MscL channel.

4. Conformation and environment of channel-forming peptides: a simulation study.

5. Functional renaturation of receptor polypeptides eluted from SDS polyacrylamide gels.

6. Orientations of amphipathic helical peptides in membrane bilayers determined by solid-state NMR spectroscopy.

Review 7. Structure and function of channels and channelogs as studied by computational chemistry.

8. X-ray scattering with momentum transfer in the plane of membrane. Application to gramicidin organization.

9. The pore domain of the nicotinic acetylcholine receptor: molecular modeling, pore dimensions, and electrostatics.

10. Molecular dynamics simulations of homo-oligomeric bundles embedded within a lipid bilayer.