Literature DB >> 2460337

Quantitative keratinocyte assay detects two biological activities of human papillomavirus DNA and identifies viral types associated with cervical carcinoma.

R Schlegel1, W C Phelps, Y L Zhang, M Barbosa.   

Abstract

Keratinocytes electroporated with human papillomavirus (HPV) DNA (HPV-6, 11, 16 and 18) exhibited an increased cellular proliferation which was quantitated as microcolony and macrocolony formation. However, only macrocolonies induced by HPV-16 or HPV-18 DNA (the two viral types most commonly found in human cervical carcinomas) gave rise to proliferating, poorly-stratified colonies when grown in the presence of serum and calcium. Hydrocortisone increased the frequency of these differentiation-resistant colonies, and studies showed that they were immortalized, contained one copy of viral DNA per cell, expressed three discrete species of viral RNA and synthesized the viral E7 protein. HPV-induced cellular proliferation and altered differentiation are therefore separable events and may represent the activity of different viral genes.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 2460337      PMCID： PMC454709          DOI： 10.1002/j.1460-2075.1988.tb03185.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  36 in total

1.  Reversible inhibition of normal human prokeratinocyte proliferation by type beta transforming growth factor-growth inhibitor in serum-free medium.

Authors:  G D Shipley; M R Pittelkow; J J Wille; R E Scott; H L Moses
Journal:  Cancer Res       Date:  1986-04       Impact factor: 12.701

2.  E5 open reading frame of bovine papillomavirus type 1 encodes a transforming gene.

Authors:  J T Schiller; W C Vass; K H Vousden; D R Lowy
Journal:  J Virol       Date:  1986-01       Impact factor: 5.103

3.  Translation of open reading frame E5 of bovine papillomavirus is required for its transforming activity.

Authors:  D DiMaio; D Guralski; J T Schiller
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

4.  Biology and biochemistry of papillomaviruses.

Authors:  H Pfister
Journal:  Rev Physiol Biochem Pharmacol       Date:  1984       Impact factor: 5.545

5.  Bovine papillomavirus contains multiple transforming genes.

Authors:  Y C Yang; H Okayama; P M Howley
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

6.  Structure and transcription of human papillomavirus sequences in cervical carcinoma cells.

Authors:  E Schwarz; U K Freese; L Gissmann; W Mayer; B Roggenbuck; A Stremlau; H zur Hausen
Journal:  Nature       Date:  1985 Mar 7-13       Impact factor: 49.962

7.  Human papillomavirus type 16 DNA-induced malignant transformation of NIH 3T3 cells.

Authors:  S Yasumoto; A L Burkhardt; J Doniger; J A DiPaolo
Journal:  J Virol       Date:  1986-02       Impact factor: 5.103

8.  Genetic analysis of the 3' early region transformation and replication functions of bovine papillomavirus type 1.

Authors:  D E Groff; W D Lancaster
Journal:  Virology       Date:  1986-04-15       Impact factor: 3.616

9.  Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.

Authors:  H Potter; L Weir; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

10.  Dissociation of transforming and trans-activation functions for bovine papillomavirus type 1.

Authors:  Y C Yang; B A Spalholz; M S Rabson; P M Howley
Journal:  Nature       Date:  1985 Dec 12-18       Impact factor: 49.962
View more
  99 in total

1.  Immortalization of primary human keratinocytes by the helix-loop-helix protein, Id-1.

Authors:  R M Alani; J Hasskarl; M Grace; M C Hernandez; M A Israel; K Münger
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-17       Impact factor: 11.205

2.  Transcription of the E6 and E7 genes of human papillomavirus type 6 in anogenital condylomata is restricted to undifferentiated cell layers of the epithelium.

Authors:  T Iftner; M Oft; S Böhm; S P Wilczynski; H Pfister
Journal:  J Virol       Date:  1992-08       Impact factor: 5.103

3.  General primer polymerase chain reaction in combination with sequence analysis for identification of potentially novel human papillomavirus genotypes in cervical lesions.

Authors:  A J van den Brule; P J Snijders; P M Raaphorst; H F Schrijnemakers; H Delius; L Gissmann; C J Meijer; J M Walboomers
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

4.  Two AP1 sites binding JunB are essential for human papillomavirus type 18 transcription in keratinocytes.

Authors:  F Thierry; G Spyrou; M Yaniv; P Howley
Journal:  J Virol       Date:  1992-06       Impact factor: 5.103

Review 5.  Cellular transformation by human papillomaviruses: lessons learned by comparing high- and low-risk viruses.

Authors:  Aloysius J Klingelhutz; Ann Roman
Journal:  Virology       Date:  2012-01-27       Impact factor: 3.616

6.  The human papillomavirus type 16 E5 oncoprotein inhibits epidermal growth factor trafficking independently of endosome acidification.

Authors:  Frank A Suprynowicz; Ewa Krawczyk; Jess D Hebert; Sawali R Sudarshan; Vera Simic; Christopher M Kamonjoh; Richard Schlegel
Journal:  J Virol       Date:  2010-08-04       Impact factor: 5.103

7.  Inactivation of both the retinoblastoma tumor suppressor and p21 by the human papillomavirus type 16 E7 oncoprotein is necessary to inhibit cell cycle arrest in human epithelial cells.

Authors:  Anna-Marija Helt; Jens Oliver Funk; Denise A Galloway
Journal:  J Virol       Date:  2002-10       Impact factor: 5.103

8.  Insulin-like growth factor-binding protein 3 expression increases during immortalization of cervical keratinocytes by human papillomavirus type 16 E6 and E7 proteins.

Authors:  Allison J Berger; Astrid Baege; Tracy Guillemette; James Deeds; Ron Meyer; Gary Disbrow; Richard Schlegel; Robert Schlegel
Journal:  Am J Pathol       Date:  2002-08       Impact factor: 4.307

9.  Human papillomavirus type 18 E7 protein requires intact Cys-X-X-Cys motifs for zinc binding, dimerization, and transformation but not for Rb binding.

Authors:  M C McIntyre; M G Frattini; S R Grossman; L A Laimins
Journal:  J Virol       Date:  1993-06       Impact factor: 5.103

10.  In vitro biological activities of the E6 and E7 genes vary among human papillomaviruses of different oncogenic potential.

Authors:  M S Barbosa; W C Vass; D R Lowy; J T Schiller
Journal:  J Virol       Date:  1991-01       Impact factor: 5.103
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.