Literature DB >> 24592106

β-human chorionic gonadotropin assay in vaginal washing fluid for the accurate diagnosis of premature rupture of membranes during late pregnancy.

Orhan Temel1, Ebru Cöğendez1, Selçuk Selçuk1, Mehmet Reşit Asoğlu2, Erdal Kaya1.   

Abstract

OBJECTIVE: To determine whether the measurement of beta-human chorionic gonadotropin (β-hCG) levels in vaginal fluid is useful for the diagnosis of premature rupture of membranes (PROM).
MATERIAL AND METHODS: A total of 92 pregnant women between 24 and 40 weeks gestation participated in this study. The patients with fluid leaking from the vagina were designated Group 1, the patients with no fluid leaking from the vagina were Group 2, and those with a suspicion of fluid leaking from the vagina were classified as Group 3. Irrigating the posterior vaginal fornix with 5 mL sterile saline was used to measure β-hCG levels of the patients. Receiver operator curve (ROC) analysis was used to determine the cut-off value for a positive diagnosis.
RESULTS: The β-hCG levels of vaginal fluid were measured as 20.5±25.0 mIU/mL, 254.6±346.8 mIU/mL, and 74.3±100.8 mIU/mL in Group 1, Group 2, and Group 3, respectively. Vaginal β-hCG level was higher statistically significantly in Group 2 than Group 1 and 3 (p<0.001). 100 mIU/mL was accepted as a cut-off value by using the receiver operating characteristic curve. According to 100 mIU/mL, sensitivity, specificity, positive predictive and negative predictive values were calculated as 71.2, 100, 100, and 65.1%, respectively.
CONCLUSION: The study showed that the measurement of β-hCG level in vaginal washing fluid is an efficient and easy diagnostic test for predicting the amount of fluid leaking from the vagina. However, due to the low negative predictive value of the test, it would not be convenient in daily practice.

Entities:  

Keywords:  Beta-human chorionic gonadotropin; diagnosis; premature rupture of membranes

Year:  2013        PMID: 24592106      PMCID: PMC3935535          DOI: 10.5152/jtgga.2013.65624

Source DB:  PubMed          Journal:  J Turk Ger Gynecol Assoc        ISSN: 1309-0380


  15 in total

1.  Vaginal fluid hCG levels for detecting premature rupture of membranes.

Authors:  T Anai; Y Tanaka; Y Hirota; I Miyakawa
Journal:  Obstet Gynecol       Date:  1997-02       Impact factor: 7.661

2.  Vaginal fluid prolactin: a reliable marker for the diagnosis of prematurely ruptured membranes. Comparison with vaginal fluid alpha-fetoprotein and placental lactogen.

Authors:  I Phocas; A Sarandakou; A Kontoravdis; A Chryssicopoulos; P A Zourlas
Journal:  Eur J Obstet Gynecol Reprod Biol       Date:  1989-05       Impact factor: 2.435

3.  Diagnosis of ruptured fetal membranes. Clinical study and review of the literature.

Authors:  M L Friedman; T W McElin
Journal:  Am J Obstet Gynecol       Date:  1969-06-15       Impact factor: 8.661

4.  Premature rupture of the membranes: the enigma of the obstetrician.

Authors:  T J Garite
Journal:  Am J Obstet Gynecol       Date:  1985-04-15       Impact factor: 8.661

5.  Use of the nitrazine yellow swab test in the diagnosis of ruptured membranes.

Authors:  A M Mills; D B Garrioch
Journal:  Br J Obstet Gynaecol       Date:  1977-02

Review 6.  Premature rupture of the membranes.

Authors:  R S Gibbs; J D Blanco
Journal:  Obstet Gynecol       Date:  1982-12       Impact factor: 7.661

7.  [Proposal of a new test for the diagnosis of PROM based on the determination of hCG in the washing fluid of the posterior vaginal fornix].

Authors:  B Mangano; F Diani; G Faccini; N Zatti; E Zardini
Journal:  Minerva Ginecol       Date:  2000-05

8.  Diagnosis of premature rupture of membranes by identification of beta-HCG in vaginal washing fluid.

Authors:  Esra Esim; Cem Turan; Orhan Unal; Ramazan Dansuk; Birol Cengizglu
Journal:  Eur J Obstet Gynecol Reprod Biol       Date:  2003-03-26       Impact factor: 2.435

9.  Dynamics of human chorionic gonadotropin, prolactin, and growth hormone in serum and amniotic fluid throughout normal human pregnancy.

Authors:  O A Kletzky; F Rossman; S I Bertolli; L D Platt; D R Mishell
Journal:  Am J Obstet Gynecol       Date:  1985-04-01       Impact factor: 8.661

10.  Diagnosis of premature rupture of the membranes by the identification of alpha-feto-protein in vaginal secretions.

Authors:  P Gaucherand; S Guibaud; R C Rudigoz; A Wong
Journal:  Acta Obstet Gynecol Scand       Date:  1994-07       Impact factor: 3.636

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