| Literature DB >> 24590790 |
Abstract
mRNA alternative polyadenylation (APA) has been increasingly recognized as a widespread and evolutionarily conserved mechanism for eukaryotic gene regulation. Here we describe a method called poly(A) site sequencing that can not only map RNA polyadenylation sites on a transcriptome level but also provide quantitative information on the relative abundance of polyadenylated RNAs. This method has been successfully used for both global APA analysis and digital gene expression profiling.Entities:
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Year: 2014 PMID： 24590790 PMCID： PMC4412162 DOI： 10.1007/978-1-62703-971-0_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745