Literature DB >> 24590717

Screening proteins for NMR suitability.

Adelinda A Yee1, Anthony Semesi, Maite Garcia, Cheryl H Arrowsmith.   

Abstract

NMR spectroscopy is a valuable tool in structural genomics. Identification of protein samples that are amenable to structure determination by NMR spectroscopy requires efficient screening. The preparation of multiple samples in parallel and screening by NMR is described. The method described is applicable to large structural genomics projects but can easily be scaled down for application to small structural biology projects. All the equipment used is commonly found in any NMR structural biology laboratory.

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Year:  2014        PMID： 24590717      PMCID： PMC4113955          DOI： 10.1007/978-1-4939-0354-2_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

1.  An NMR approach to structural proteomics.

Authors:  Adelinda Yee; Xiaoqing Chang; Antonio Pineda-Lucena; Bin Wu; Anthony Semesi; Brian Le; Theresa Ramelot; Gregory M Lee; Sudeepa Bhattacharyya; Pablo Gutierrez; Aleksej Denisov; Chang-Hun Lee; John R Cort; Guennadi Kozlov; Jack Liao; Grzegorz Finak; Limin Chen; David Wishart; Weontae Lee; Lawrence P McIntosh; Kalle Gehring; Michael A Kennedy; Aled M Edwards; Cheryl H Arrowsmith
Journal:  Proc Natl Acad Sci U S A       Date:  2002-02-19       Impact factor: 11.205

2.  Structural proteomics of an archaeon.

Authors:  D Christendat; A Yee; A Dharamsi; Y Kluger; A Savchenko; J R Cort; V Booth; C D Mackereth; V Saridakis; I Ekiel; G Kozlov; K L Maxwell; N Wu; L P McIntosh; K Gehring; M A Kennedy; A R Davidson; E F Pai; M Gerstein; A M Edwards; C H Arrowsmith
Journal:  Nat Struct Biol       Date:  2000-10

3.  NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors:  F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal:  J Biomol NMR       Date:  1995-11       Impact factor: 2.835

4.  Simple defined autoinduction medium for high-level recombinant protein production using T7-based Escherichia coli expression systems.

Authors:  Zhaopeng Li; Wolfgang Kessler; Joop van den Heuvel; Ursula Rinas
Journal:  Appl Microbiol Biotechnol       Date:  2011-06-23       Impact factor: 4.813

5.  Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.

Authors:  F W Studier; B A Moffatt
Journal:  J Mol Biol       Date:  1986-05-05       Impact factor: 5.469

6.  NleG Type 3 effectors from enterohaemorrhagic Escherichia coli are U-Box E3 ubiquitin ligases.

Authors:  Bin Wu; Tatiana Skarina; Adelinda Yee; Marie-Claude Jobin; Rosa Dileo; Anthony Semesi; Christophe Fares; Alexander Lemak; Brian K Coombes; Cheryl H Arrowsmith; Alexander U Singer; Alexei Savchenko
Journal:  PLoS Pathog       Date:  2010-06-24       Impact factor: 6.823

7.  NMR for structural proteomics of Thermotoga maritima: screening and structure determination.

Authors:  Wolfgang Peti; Touraj Etezady-Esfarjani; Torsten Herrmann; Heath E Klock; Scott A Lesley; Kurt Wüthrich
Journal:  J Struct Funct Genomics       Date:  2004

8.  Inducer exclusion by glucose 6-phosphate in Escherichia coli.

Authors:  B M Hogema; J C Arents; R Bader; K Eijkemans; T Inada; H Aiba; P W Postma
Journal:  Mol Microbiol       Date:  1998-05       Impact factor: 3.501

9.  Myxoma virus immunomodulatory protein M156R is a structural mimic of eukaryotic translation initiation factor eIF2alpha.

Authors:  Theresa A Ramelot; John R Cort; Adelinda A Yee; Furong Liu; Michael B Goshe; Aled M Edwards; Richard D Smith; Cheryl H Arrowsmith; Thomas E Dever; Michael A Kennedy
Journal:  J Mol Biol       Date:  2002-10-04       Impact factor: 5.469

10.  Unique opportunities for NMR methods in structural genomics.

Authors:  Gaetano T Montelione; Cheryl Arrowsmith; Mark E Girvin; Michael A Kennedy; John L Markley; Robert Powers; James H Prestegard; Thomas Szyperski
Journal:  J Struct Funct Genomics       Date:  2009-03-15
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  1 in total

1.  Enzyme-Triggered Dissociation of a FRET-Based Protein Biosensor Monitored by Synchrotron SAXS.

Authors:  Greta Faccio; Stefan Salentinig
Journal:  Biophys J       Date:  2017-10-17       Impact factor: 4.033
  1 in total

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