Masakazu Nakamura1, Yuzo Kayamori2, Hiroyasu Iso3, Akihiko Kitamura4, Masahiko Kiyama4, Isao Koyama5, Kunihiro Nishimura6, Michikazu Nakai7, Hiroyuki Noda8, Mahnaz Dasti9, Hubert W Vesper9, Yoshihiro Miyamoto10. 1. National Cerebral and Cardiovascular Center, Department of Preventive Cardiology, Lipid Reference Laboratory, Japan. Electronic address: nakamura.masakazu.hp@ncvc.go.jp. 2. Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, Japan. 3. Public Health, Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Japan. 4. Osaka Center for Cancer and Cardiovascular Disease Prevention, Japan. 5. National Cerebral and Cardiovascular Center, Department of Preventive Cardiology, Lipid Reference Laboratory, Japan. 6. National Cerebral and Cardiovascular Center, Department of Preventive Medicine and Epidemiologic Informatics, Office of Evidence-based Medicine and Risk Analysis, Japan. 7. National Cerebral and Cardiovascular Center, Department of Preventive Medicine and Epidemiologic Informatics, Japan. 8. Public Health, Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Japan; Ministry of Health, Labour and Welfare, Health Service Bureau, Cancer Control and Health Promotion Division, Japan. 9. Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, USA. 10. National Cerebral and Cardiovascular Center, Department of Preventive Cardiology, Department of Preventive Medicine and Epidemiologic Informatics, Japan.
Abstract
BACKGROUND: Accurate measurement of blood lipids is crucial in cardiovascular disease risk management. The Centers for Disease Control and Prevention (CDC) Cholesterol Reference Method Laboratory Network (CRMLN) has assured the accuracy of these measurements for over 20 years using beta quantification (BQ) method as reference measurement procedure (RMP) for high- and low-density lipoprotein cholesterol (HDL-C, LDL-C). Only limited data exist about the performance of the BQ RMP. METHODS: Bottom fraction cholesterol (BFC), HDL-C, and LDL-C results after ultracentrifugation from the CDC lipid reference laboratory and the Japanese CRMLN laboratory were compared using 280 serum samples measured over the past 15 years. Data were compared statistically using method comparison and bias estimation analysis. RESULTS: Regression analysis between CDC (x) and Osaka (y) for BFC, HDL-C, and LDL-C were y=0.988x+1.794 (R(2)=0.997), y=0.980x+1.118 (R(2)=0.994), and y=0.987x+1.200 (R(2)=0.997), respectively. The Osaka laboratory met performance goals for 90% to 95% of the CDC reference values. CONCLUSIONS: The BQ method by the Osaka CRMLN laboratory is highly accurate and has been stable for over 15 years. Accurate measurement of BFC is critical for the determination of LDL-C.
BACKGROUND: Accurate measurement of blood lipids is crucial in cardiovascular disease risk management. The Centers for Disease Control and Prevention (CDC) Cholesterol Reference Method Laboratory Network (CRMLN) has assured the accuracy of these measurements for over 20 years using beta quantification (BQ) method as reference measurement procedure (RMP) for high- and low-density lipoprotein cholesterol (HDL-C, LDL-C). Only limited data exist about the performance of the BQ RMP. METHODS: Bottom fraction cholesterol (BFC), HDL-C, and LDL-C results after ultracentrifugation from the CDC lipid reference laboratory and the Japanese CRMLN laboratory were compared using 280 serum samples measured over the past 15 years. Data were compared statistically using method comparison and bias estimation analysis. RESULTS: Regression analysis between CDC (x) and Osaka (y) for BFC, HDL-C, and LDL-C were y=0.988x+1.794 (R(2)=0.997), y=0.980x+1.118 (R(2)=0.994), and y=0.987x+1.200 (R(2)=0.997), respectively. The Osaka laboratory met performance goals for 90% to 95% of the CDC reference values. CONCLUSIONS: The BQ method by the Osaka CRMLN laboratory is highly accurate and has been stable for over 15 years. Accurate measurement of BFC is critical for the determination of LDL-C.
Authors: Thomas G Cole; John H Contois; Gyorgy Csako; Joseph P McConnell; Alan T Remaley; Sridevi Devaraj; Daniel M Hoefner; Tonya Mallory; Amar A Sethi; G Russell Warnick Journal: Clin Chem Date: 2013-02-05 Impact factor: 8.327