| Literature DB >> 24582836 |
Chi-Chiu Lau1, Tingting Sun1, Arthur K K Ching1, Mian He1, Jing-Woei Li2, Alissa M Wong1, Ngai Na Co1, Anthony W H Chan1, Pik-Shan Li3, Raymond W M Lung1, Joanna H M Tong1, Paul B S Lai4, Henry L Y Chan5, Ka-Fai To6, Ting-Fung Chan7, Nathalie Wong8.
Abstract
The mutagenic effect of hepatitis B (HBV) integration in predisposing risk to hepatocellular carcinoma (HCC) remains elusive. In this study, we performed transcriptome sequencing of HBV-positive HCC cell lines and showed transcription of viral-human gene fusions from the site of genome integrations. We discovered tumor-promoting properties of a chimeric HBx-LINE1 that, intriguingly, functions as a hybrid RNA. HBx-LINE1 can be detected in 23.3% of HBV-associated HCC tumors and correlates with poorer patient survival. HBx-LINE1 transgenic mice showed heightened susceptibility to diethylnitrosamine-induced tumor formation. We further show that HBx-LINE1 expression affects β-catenin transactivity, which underlines a role in activating Wnt signaling. Thus, this study identifies a viral-human chimeric fusion transcript that functions like a long noncoding RNA to promote HCC.Entities:
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Year: 2014 PMID： 24582836 DOI： 10.1016/j.ccr.2014.01.030
Source DB: PubMed Journal: Cancer Cell ISSN： 1535-6108 Impact factor: 31.743