Ji Young Kim1, Eun Jae Kim1, Myung Jae Jeon2, Ran Kim1, Min Woo Lee3, Suhng Wook Kim4. 1. Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea. 2. Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea. Electronic address: jeonmj@snu.ac.kr. 3. Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea; Department of Biomedical Science, College of Health Sciences, Korea University, Seoul, Republic of Korea. 4. Department of Biomedical Science, College of Health Sciences, Korea University, Seoul, Republic of Korea.
Abstract
OBJECTIVE: Oxidative stress is associated with the pathogenesis of pelvic organ prolapse (POP). Because glutathione S-transferases (GSTs) are the major detoxification enzymes which protect cells against oxidative stress, genetic variations in the GST gene may modulate the risk of POP. This study aimed to determine the association between advanced POP and the polymorphisms of GSTM1, GSTT1 and GSTP1 (rs1695). STUDY DESIGN: This is a hospital-based case-control study. The POP group consisted of 189 women diagnosed with POP stage III or IV, and the control group consisted of 156 postmenopausal women with POP stage 0 or I. The GSTM1 and GSTT1 null mutations were detected by multiplex PCR, and the GSTP1 Ile105Val polymorphism was genotyped by real-time PCR analysis using a TaqMan assay. RESULTS: There was no significant association between the GSTM1 and GSTT1 null mutations and advanced POP (p>0.05). The distribution of the GSTP1 Ile105Val genotypes, however, was significantly different between the POP and control groups (AA/AG/GG rates=74.1%/25.9%/0% vs. 64.1%/32.1%/3.8%, p=0.008), and the G allele frequency was significantly lower in the POP group than in the control group (13.0% vs. 19.9%, p=0.014). Women with the non-AA genotype had a 0.63-fold lower risk of developing advanced POP than women with the AA genotype (95% CI, 0.39-0.99), and women with the G allele had a 0.60-fold lower risk of advanced POP than women with the A allele (95% CI, 0.40-0.90). CONCLUSIONS: The GSTP1 Ile105Val polymorphism is a protective factor against advanced POP.
OBJECTIVE: Oxidative stress is associated with the pathogenesis of pelvic organ prolapse (POP). Because glutathione S-transferases (GSTs) are the major detoxification enzymes which protect cells against oxidative stress, genetic variations in the GST gene may modulate the risk of POP. This study aimed to determine the association between advanced POP and the polymorphisms of GSTM1, GSTT1 and GSTP1 (rs1695). STUDY DESIGN: This is a hospital-based case-control study. The POP group consisted of 189 women diagnosed with POP stage III or IV, and the control group consisted of 156 postmenopausal women with POP stage 0 or I. The GSTM1 and GSTT1 null mutations were detected by multiplex PCR, and the GSTP1Ile105Val polymorphism was genotyped by real-time PCR analysis using a TaqMan assay. RESULTS: There was no significant association between the GSTM1 and GSTT1 null mutations and advanced POP (p>0.05). The distribution of the GSTP1Ile105Val genotypes, however, was significantly different between the POP and control groups (AA/AG/GG rates=74.1%/25.9%/0% vs. 64.1%/32.1%/3.8%, p=0.008), and the G allele frequency was significantly lower in the POP group than in the control group (13.0% vs. 19.9%, p=0.014). Women with the non-AA genotype had a 0.63-fold lower risk of developing advanced POP than women with the AA genotype (95% CI, 0.39-0.99), and women with the G allele had a 0.60-fold lower risk of advanced POP than women with the A allele (95% CI, 0.40-0.90). CONCLUSIONS: The GSTP1Ile105Val polymorphism is a protective factor against advanced POP.
Authors: Rufus Cartwright; Anna C Kirby; Kari A O Tikkinen; Altaf Mangera; Gans Thiagamoorthy; Prabhakar Rajan; Jori Pesonen; Chris Ambrose; Juan Gonzalez-Maffe; Phillip Bennett; Tom Palmer; Andrew Walley; Marjo-Riitta Järvelin; Chris Chapple; Vik Khullar Journal: Am J Obstet Gynecol Date: 2014-08-08 Impact factor: 8.661
Authors: Kristina Allen-Brady; John W F Chua; Romana Cuffolo; Marianne Koch; Felice Sorrentino; Rufus Cartwright Journal: Int Urogynecol J Date: 2021-04-24 Impact factor: 1.932