S Abbaszadeh1, A Sharifzadeh2, H Shokri3, A R Khosravi2, A Abbaszadeh4. 1. Baqiyatallah university of medical sciences, Tehran, Iran. 2. Mycology research center, faculty of veterinary medicine, university of Tehran, Tehran, Iran. 3. Faculty of veterinary medicine, Amol university of special modern technologies, 24th aftab, Imam Khomeini street, Amol, Iran. Electronic address: hshokri@umz.ac.ir. 4. Kermanshah university of medical sciences, Kermanshah, Iran.
Abstract
OBJECTIVE: This work is an attempt to examine the antifungal activity of thymol, carvacrol, eugenol and menthol against 11 food-decaying fungi. METHODS: The susceptibility test for the compounds was carried out in terms of minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) using microdilution method in 96 multi-well microtiter plates. RESULTS: Results indicated that all compounds were effective to varying extents against various fungal isolates, with the highest efficacy displayed by carvacrol (mean MIC value: 154.5 μg/mL) (P<0.05). The incorporation of increased concentrations of all compounds to the media led to progressive and significant reduction in growth for all fungi. The most potent inhibitory activity of thymol, carvacrol, eugenol and menthol was found for Cladosporium spp. (MIC: 100 μg/mL), Aspergillus spp. (MIC: 100 μg/mL), Cladosporium spp. (MIC: 350 μg/mL), and Aspergillus spp. and Cladosporium spp. (MIC: 125 μg/mL), respectively. CONCLUSION: Thus, the application of these herbal components could be considered as a good alternatives to inhibit fungal growth and to reduce the use of synthetic fungicides.
OBJECTIVE: This work is an attempt to examine the antifungal activity of thymol, carvacrol, eugenol and menthol against 11 food-decaying fungi. METHODS: The susceptibility test for the compounds was carried out in terms of minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) using microdilution method in 96 multi-well microtiter plates. RESULTS: Results indicated that all compounds were effective to varying extents against various fungal isolates, with the highest efficacy displayed by carvacrol (mean MIC value: 154.5 μg/mL) (P<0.05). The incorporation of increased concentrations of all compounds to the media led to progressive and significant reduction in growth for all fungi. The most potent inhibitory activity of thymol, carvacrol, eugenol and menthol was found for Cladosporium spp. (MIC: 100 μg/mL), Aspergillus spp. (MIC: 100 μg/mL), Cladosporium spp. (MIC: 350 μg/mL), and Aspergillus spp. and Cladosporium spp. (MIC: 125 μg/mL), respectively. CONCLUSION: Thus, the application of these herbal components could be considered as a good alternatives to inhibit fungal growth and to reduce the use of synthetic fungicides.
Authors: Jonathan Delgado-Adámez; María Garrido; Maria Elena Bote; María C Fuentes-Pérez; Javier Espino; Daniel Martín-Vertedor Journal: J Food Sci Technol Date: 2017-04-10 Impact factor: 2.701
Authors: Francisco Felipe Maia da Silva; Francisco José Queiroz Monte; Telma Leda Gomes de Lemos; Patrícia Georgina Garcia do Nascimento; Alana Kelly de Medeiros Costa; Luanda Misley Mota de Paiva Journal: Chem Cent J Date: 2018-04-03 Impact factor: 4.215