| Literature DB >> 24581436 |
Abstract
Eukaryotic cell-free in vitro translation systems have been in use since the 1970s. These systems can faithfully synthesize polypeptides when programmed with mRNA, enabling the production of polypeptides for analysis as well as permitting analyses of the cis- and trans-acting factors that regulate translation. Here we describe the preparation and use of cell-free translation systems from the yeast Saccharomyces cerevisiae.Entities:
Keywords: Amino acids; Cell-free translation; Chromatography; Dithiothreitol (DTT) and phenylmethylsulfonyl fluoride (PMSF); Preparation of yeast cell-free extract; Radiolabeled translation products; Renilla luciferase mRNA; Room temperature; Saccharomyces Cerevisiae cells
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Year: 2014 PMID： 24581436 DOI： 10.1016/B978-0-12-420120-0.00002-5
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600