PURPOSE: To investigate the presence of anti-angiotensin converting enzyme (ACE) factors in aqueous extract of tomato. METHODS: The bio-guided fractionation of the aqueous extract of tomato produced a sugar-free, heat-stable fraction with molecular mass <1,000 Da from tomatoes. The sugar-free tomato extract (TE) was tested for its anti-ACE activity using human plasma and rabbit lung pure ACE. In addition, its effect on human platelet aggregation induced by ADP, collagen or arachidonic acid was determined. The mechanism of platelet inhibitory action of TE was investigated by measuring platelet factor 4 (PF4) release and cAMP synthesis by platelets. RESULTS: Typically, 100 g tomatoes produced 72.2 ± 4.7 mg of TE. This extract inhibited both platelet aggregation and plasma ACE activity in a dose-dependent manner. It inhibited platelet aggregation in response to ADP, collagen or arachidonic acid, and inhibitory action was mediated in part by reducing platelet PF4 release and by stimulating cAMP synthesis. The IC50 value of TE for ADP-induced platelet aggregation was 0.4 ± 0.02 mg/ml, whereas the IC50 value for ACE enzyme inhibition was 1.40 ± 0.04 mg/ml. Both the TE and commercially available sugar-free TE, Fruitflow(®)-2 had similar amount of catechin, and also had equal inhibitory potencies against platelet aggregation and plasma ACE activity. CONCLUSION: Together these data indicate that aqueous extract of tomatoes contain anti-ACE factors in addition to previously described anti-platelet factors.
PURPOSE: To investigate the presence of anti-angiotensin converting enzyme (ACE) factors in aqueous extract of tomato. METHODS: The bio-guided fractionation of the aqueous extract of tomato produced a sugar-free, heat-stable fraction with molecular mass <1,000 Da from tomatoes. The sugar-free tomato extract (TE) was tested for its anti-ACE activity using human plasma and rabbit lung pure ACE. In addition, its effect on humanplatelet aggregation induced by ADP, collagen or arachidonic acid was determined. The mechanism of platelet inhibitory action of TE was investigated by measuring platelet factor 4 (PF4) release and cAMP synthesis by platelets. RESULTS: Typically, 100 g tomatoes produced 72.2 ± 4.7 mg of TE. This extract inhibited both platelet aggregation and plasma ACE activity in a dose-dependent manner. It inhibited platelet aggregation in response to ADP, collagen or arachidonic acid, and inhibitory action was mediated in part by reducing platelet PF4 release and by stimulating cAMP synthesis. The IC50 value of TE for ADP-induced platelet aggregation was 0.4 ± 0.02 mg/ml, whereas the IC50 value for ACE enzyme inhibition was 1.40 ± 0.04 mg/ml. Both the TE and commercially available sugar-free TE, Fruitflow(®)-2 had similar amount of catechin, and also had equal inhibitory potencies against platelet aggregation and plasma ACE activity. CONCLUSION: Together these data indicate that aqueous extract of tomatoes contain anti-ACE factors in addition to previously described anti-platelet factors.
Authors: Niamh O'Kennedy; Lynn Crosbie; Stuart Whelan; Vanessa Luther; Graham Horgan; John I Broom; David J Webb; Asim K Duttaroy Journal: Am J Clin Nutr Date: 2006-09 Impact factor: 7.045
Authors: E Obarzanek; F M Sacks; W M Vollmer; G A Bray; E R Miller; P H Lin; N M Karanja; M M Most-Windhauser; T J Moore; J F Swain; C W Bales; M A Proschan Journal: Am J Clin Nutr Date: 2001-07 Impact factor: 7.045
Authors: Monica Rosa Loizzo; Ataa Said; Rosa Tundis; Khaled Rashed; Giancarlo Antonio Statti; Antje Hufner; Francesco Menichini Journal: Phytother Res Date: 2007-01 Impact factor: 5.878
Authors: Angelika N Osińska; Beata Begier-Krasińska; Piotr Rzymski; Aleksandra Krasińska; Andrzej Tykarski; Zbigniew Krasiński Journal: Kardiochir Torakochirurgia Pol Date: 2017-12-20
Authors: Beata Krasinska; Angelika Osińska; Maciej Osinski; Aleksandra Krasinska; Piotr Rzymski; Andrzej Tykarski; Zbigniew Krasiński Journal: Arch Med Sci Date: 2017-09-05 Impact factor: 3.318