Sibel Atalay1, Atilla Coruh2, Kemal Deniz3. 1. Private Medisu Hospital, Department of Plastic Reconstructive and Aesthetic Surgery, Antalya, Turkey. 2. Erciyes University Medical Faculty, Department of Plastic Aesthetic and Reconstructive Surgery , Kayseri, Turkey. Electronic address: atilla.coruh@superonline.com. 3. Erciyes University Medical Faculty, Department of Pathology, Kayseri, Turkey.
Abstract
OBJECTIVE: The practice of early burn wound excision and wound closure by immediate autologous skin or skin substitutes is the preferred treatment in extensive deep partial and full-thickness burns. To date there is no proven definite medical treatment to decrease burn wound size and accelerate burn wound healing in modern clinical practice. Stromal vascular fraction is an autologous mixture that has multiple proven beneficial effects on different kinds of wounds. In our study, we investigated the effects of stromal vascular fraction on deep partial-thickness burn wound healing. METHODS: In this study, 20 Wistar albino rats were used. Inguinal adipose tissue of the rats was surgically removed and stromal vascular fraction was isolated. Thereafter, deep second-degree burns were performed on the back of the rats by hot water. The rats were divided into two groups in a randomized fashion. The therapy group received stromal vascular fraction, whereas the control group received only physiologic serum by intradermal injection. Assessment of the burn wound healing between the groups was carried out by histopathologic and immuno-histochemical data. RESULTS: Stromal vascular fraction increased vascular endothelial growth factor, proliferating cell nuclear antigen index, and reduced inflammation of the burn wound. Furthermore, vascularization and fibroblastic activity were achieved earlier and observed to be at higher levels in the stromal vascular fraction group. CONCLUSIONS: Stromal vascular fraction improves burn wound healing by increasing cell proliferation and vascularization, reducing inflammation, and increasing fibroblastic activity.
OBJECTIVE: The practice of early burn wound excision and wound closure by immediate autologous skin or skin substitutes is the preferred treatment in extensive deep partial and full-thickness burns. To date there is no proven definite medical treatment to decrease burn wound size and accelerate burn wound healing in modern clinical practice. Stromal vascular fraction is an autologous mixture that has multiple proven beneficial effects on different kinds of wounds. In our study, we investigated the effects of stromal vascular fraction on deep partial-thickness burn wound healing. METHODS: In this study, 20 Wistar albino rats were used. Inguinal adipose tissue of the rats was surgically removed and stromal vascular fraction was isolated. Thereafter, deep second-degree burns were performed on the back of the rats by hot water. The rats were divided into two groups in a randomized fashion. The therapy group received stromal vascular fraction, whereas the control group received only physiologic serum by intradermal injection. Assessment of the burn wound healing between the groups was carried out by histopathologic and immuno-histochemical data. RESULTS: Stromal vascular fraction increased vascular endothelial growth factor, proliferating cell nuclear antigen index, and reduced inflammation of the burn wound. Furthermore, vascularization and fibroblastic activity were achieved earlier and observed to be at higher levels in the stromal vascular fraction group. CONCLUSIONS: Stromal vascular fraction improves burn wound healing by increasing cell proliferation and vascularization, reducing inflammation, and increasing fibroblastic activity.
Authors: Philippe Foubert; Andreina D Gonzalez; Stephan Teodosescu; Felipe Berard; Melanie Doyle-Eisele; Krishna Yekkala; Mayer Tenenhaus; John K Fraser Journal: Adv Wound Care (New Rochelle) Date: 2016-07-01 Impact factor: 4.730
Authors: José Fábio Santos Duarte Lana; Anna Vitória Santos Duarte Lana; Lucas Furtado da Fonseca; Marcelo Amaral Coelho; Guilherme Gabriel Marques; Tomas Mosaner; Lucas Leite Ribeiro; Gabriel Ohana Marques Azzini; Gabriel Silva Santos; Eduardo Fonseca; Marco Antonio Percope de Andrade Journal: J Stem Cells Regen Med Date: 2022-04-05