Literature DB >> 2456582

Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library.

J R Duguid1, R G Rohwer, B Seed.   

Abstract

We have developed a subtractive cloning procedure based on the hybridization of single-stranded cDNA libraries constructed in pi H3M, a vector containing the phage M13 origin of replication. We have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brain. DNA sequence analysis showed that they represent glial fibrillary acidic protein, metallothionein II, and the B chain of alpha-crystallin; the latter two may represent a response to stress.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 2456582      PMCID： PMC281836          DOI： 10.1073/pnas.85.15.5738

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  35 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  Unconventional viruses and the origin and disappearance of kuru.

Authors:  D C Gajdusek
Journal:  Science       Date:  1977-09-02       Impact factor: 47.728

3.  Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.

Authors:  A Aruffo; B Seed
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

4.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

5.  Brain aluminum distribution in Alzheimer's disease and experimental neurofibrillary degeneration.

Authors:  D R Crapper; S S Krishnan; A J Dalton
Journal:  Science       Date:  1973-05-04       Impact factor: 47.728

6.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

7.  Characteristics of a short incubation model of scrapie in the golden hamster.

Authors:  R H Kimberlin; C Walker
Journal:  J Gen Virol       Date:  1977-02       Impact factor: 3.891

8.  A novel method of activation of cross-linked agaroses with 1,1'-carbonyldiimidazole which gives a matrix for affinity chromatography devoid of additional charged groups.

Authors:  G S Bethell; J S Ayers; W S Hancock; M T Hearn
Journal:  J Biol Chem       Date:  1979-04-25       Impact factor: 5.157

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Complex population of nonpolyadenylated messenger RNA in mouse brain.

Authors:  J Van Ness; I H Maxwell; W E Hahn
Journal:  Cell       Date:  1979-12       Impact factor: 41.582
View more
  72 in total

Review 1.  Subtractive hybridization--genetic takeaways and the search for meaning.

Authors:  R J Byers; J A Hoyland; J Dixon; A J Freemont
Journal:  Int J Exp Pathol       Date:  2000-12       Impact factor: 1.925

2.  Alpha-crystallin and ATP facilitate the in vitro renaturation of xylanase: enhancement of refolding by metal ions.

Authors:  Devyani Nath; Urmila Rawat; Ramakrishnan Anish; Mala Rao
Journal:  Protein Sci       Date:  2002-11       Impact factor: 6.725

3.  Identification of upregulated genes in scrapie-infected brain tissue.

Authors:  C Riemer; I Queck; D Simon; R Kurth; M Baier
Journal:  J Virol       Date:  2000-11       Impact factor: 5.103

4.  Alpha-crystallin can function as a molecular chaperone.

Authors:  J Horwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1992-11-01       Impact factor: 11.205

Review 5.  A superfamily in the mammalian eye lens: the beta/gamma-crystallins.

Authors:  G L van Rens; W W de Jong; H Bloemendal
Journal:  Mol Biol Rep       Date:  1992-02       Impact factor: 2.316

6.  Identification of mRNAs associated with programmed cell death in immature thymocytes.

Authors:  G P Owens; W E Hahn; J J Cohen
Journal:  Mol Cell Biol       Date:  1991-08       Impact factor: 4.272

7.  A simple and efficient cDNA library subtraction procedure: isolation of human retina-specific cDNA clones.

Authors:  A Swaroop; J Z Xu; N Agarwal; S M Weissman
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

Review 8.  The search for scrapie agent nucleic acid.

Authors:  J M Aiken; R F Marsh
Journal:  Microbiol Rev       Date:  1990-09

9.  Reverse transcriptase. The use of cloned Moloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA.

Authors:  G F Gerard; D K Fox; M Nathan; J M D'Alessio
Journal:  Mol Biotechnol       Date:  1997-08       Impact factor: 2.695

10.  A novel blue light- and abscisic acid-inducible gene of Arabidopsis thaliana encoding an intrinsic membrane protein.

Authors:  R Kaldenhoff; A Kölling; G Richter
Journal:  Plant Mol Biol       Date:  1993-12       Impact factor: 4.076
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.