Belinda J Hales1, Lee Ying Chai2, Lee Hazell2, Claire E Elliot2, Shane Stone2, Serena E O'Neil2, Wendy-Anne Smith2, Wayne R Thomas2. 1. Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, Subiaco Wa, Australia. Electronic address: belinda@ichr.uwa.edu.au. 2. Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, Subiaco Wa, Australia.
Abstract
BACKGROUND: Cat allergy affects approximately 15% of the population and is a major risk factor for asthma. The relative importance of cat allergens other than Fel d 1 is not known. OBJECTIVE: To compare IgE and IgG antibody binding and T-cell recognition of the major cat allergen Fel d 1 with other cat proteins with known IgE binding properties. METHODS: IgE, IgG1, and IgG4 antibody to Fel d 1, 2, 3, 4, 7, 8, and the undesignated IgE binding proteins haptoglobin and S100A12 were measured in the plasma of 96 individuals with cat allergy and 78 individuals without cat allergy. Cytokines were measured from T cells stimulated with the cat allergens. RESULTS: An allergen other than Fel d 1 had the highest IgE binding specificity for 35% of individuals with cat allergy, and it bound more than 50% of their IgE antibody in 70% of these sera. Fel d 4, 7, and 8 were identified as the main contributors to the non-Fel d 1 IgE binding response and elicited inflammatory Th2 cytokines to a similar degree as Fel d 1. As expected, the average percentage of IgE binding to Fel d 1 for individuals was 55%. IgG4 binding to Fel d 1 was detected in both subjects with allergy (30%) and subjects without allergy (19%). IgG4 binding to the other allergens was less prevalent but was found for both groups. IgG1 antibody was not detected to any of the newly described cat proteins. CONCLUSION: Fel d 4, 7, and 8 are allergens that should be included in the diagnosis and investigation of cat allergy.
BACKGROUND: Cat allergy affects approximately 15% of the population and is a major risk factor for asthma. The relative importance of cat allergens other than Fel d 1 is not known. OBJECTIVE: To compare IgE and IgG antibody binding and T-cell recognition of the major cat allergen Fel d 1 with other cat proteins with known IgE binding properties. METHODS: IgE, IgG1, and IgG4 antibody to Fel d 1, 2, 3, 4, 7, 8, and the undesignated IgE binding proteins haptoglobin and S100A12 were measured in the plasma of 96 individuals with cat allergy and 78 individuals without cat allergy. Cytokines were measured from T cells stimulated with the cat allergens. RESULTS: An allergen other than Fel d 1 had the highest IgE binding specificity for 35% of individuals with cat allergy, and it bound more than 50% of their IgE antibody in 70% of these sera. Fel d 4, 7, and 8 were identified as the main contributors to the non-Fel d 1 IgE binding response and elicited inflammatory Th2 cytokines to a similar degree as Fel d 1. As expected, the average percentage of IgE binding to Fel d 1 for individuals was 55%. IgG4 binding to Fel d 1 was detected in both subjects with allergy (30%) and subjects without allergy (19%). IgG4 binding to the other allergens was less prevalent but was found for both groups. IgG1 antibody was not detected to any of the newly described cat proteins. CONCLUSION: Fel d 4, 7, and 8 are allergens that should be included in the diagnosis and investigation of cat allergy.
Authors: Thomas A E Platts-Mills; Alexander J Schuyler; Elizabeth A Erwin; Scott P Commins; Judith A Woodfolk Journal: J Allergy Clin Immunol Date: 2016-04-27 Impact factor: 10.793
Authors: Amedee Renand; Luis D Archila; John McGinty; Erik Wambre; David Robinson; Belinda J Hales; Wayne R Thomas; William W Kwok Journal: J Allergy Clin Immunol Date: 2015-09-11 Impact factor: 10.793
Authors: Anna Asarnoj; Carl Hamsten; Konrad Wadén; Christian Lupinek; Niklas Andersson; Inger Kull; Mirela Curin; Josep Anto; Jean Bousquet; Rudolf Valenta; Magnus Wickman; Marianne van Hage Journal: J Allergy Clin Immunol Date: 2015-12-10 Impact factor: 10.793